A Reproducible Method for Isolation and In Vitro Culture of Functional Human Lymphoid Stromal Cells from Tonsils

Open Access

Peer-reviewed

Research Article

Yotam E. Bar-Ephraim,

Affiliation Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, The Netherlands
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Tanja Konijn,

Affiliation Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, The Netherlands
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Mehmet Gönültas,

Affiliation Department of Otolaryngology, MC Hospital, Amsterdam, The Netherlands
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Reina E. Mebius,

Affiliation Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, The Netherlands
⨯
Rogier M. Reijmers

* E-mail: r.reijmers@vumc.nl

Affiliation Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, The Netherlands

http://orcid.org/0000-0001-7471-7715

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Tonsils
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Stromal cells
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Cell staining
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Cytokines
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Lymphocytes
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Immune response
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Flow cytometry
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T cells
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