A Nested PCR Assay to Avoid False Positive Detection of the Microsporidian Enterocytozoon hepatopenaei (EHP) in Environmental Samples in Shrimp Farms

Pattana Jaroenlak; Piyachat Sanguanrut; Bryony A. P. Williams; Grant D. Stentiford; Timothy W. Flegel; Kallaya Sritunyalucksana; Ornchuma Itsathitphaisarn

doi:10.1371/journal.pone.0166320

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Open Access

Peer-reviewed

Research Article

A Nested PCR Assay to Avoid False Positive Detection of the Microsporidian Enterocytozoon hepatopenaei (EHP) in Environmental Samples in Shrimp Farms

Pattana Jaroenlak,

Affiliations Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand, Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol University, Bangkok, Thailand
⨯
Piyachat Sanguanrut,

Affiliations Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol University, Bangkok, Thailand, Shrimp Pathogen Interaction Laboratory (SPI), National Center for Genetic Engineering and Biotechnology (BIOTEC), Bangkok, Thailand
⨯
Bryony A. P. Williams,

Affiliation Biosciences, College of Life and Environmental Sciences, University of Exeter, Exeter, United Kingdom
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Grant D. Stentiford,

Affiliation European Community Reference Laboratory for Crustacean Diseases, Center for Environment, Fisheries and Aquaculture Science (Cefas), Weymouth, Dorset, United Kingdom
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Timothy W. Flegel,

Affiliations Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol University, Bangkok, Thailand, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, Thailand
⨯
Kallaya Sritunyalucksana,

Affiliations Shrimp Pathogen Interaction Laboratory (SPI), National Center for Genetic Engineering and Biotechnology (BIOTEC), Bangkok, Thailand, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, Thailand
⨯
Ornchuma Itsathitphaisarn

* E-mail: ornchuma.its@mahidol.ac.th

Affiliations Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand, Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol University, Bangkok, Thailand

http://orcid.org/0000-0003-0371-3877

⨯

A Nested PCR Assay to Avoid False Positive Detection of the Microsporidian Enterocytozoon hepatopenaei (EHP) in Environmental Samples in Shrimp Farms

Pattana Jaroenlak,
Piyachat Sanguanrut,
Bryony A. P. Williams,
Grant D. Stentiford,
Timothy W. Flegel,
Kallaya Sritunyalucksana,
Ornchuma Itsathitphaisarn

Published: November 10, 2016
https://doi.org/10.1371/journal.pone.0166320

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Polymerase chain reaction
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Shrimp
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Microsporidia
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Ribosomal RNA
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Sequence alignment
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Nested polymerase chain reaction
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