Ribozyme Mediated gRNA Generation for In Vitro and In Vivo CRISPR/Cas9 Mutagenesis

Open Access

Peer-reviewed

Research Article

Raymond Teck Ho Lee ,

* E-mail: raymondlee@duke-nus.edu.sg (RTHL); pingham@ntu.edu.sg (PWI)

Current address: Program in Cancer and Stem Cell Biology, Duke-NUS Graduate Medical School, Singapore, Singapore

Affiliation Developmental and Biomedical Genetics Laboratory, Institute of Molecular and Cell biology, Agency of Science, Technology and Research (A-STAR), Singapore

http://orcid.org/0000-0001-6522-3043

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Ashley Shu Mei Ng,

Affiliation Developmental and Biomedical Genetics Laboratory, Institute of Molecular and Cell biology, Agency of Science, Technology and Research (A-STAR), Singapore
⨯
Philip W. Ingham

* E-mail: raymondlee@duke-nus.edu.sg (RTHL); pingham@ntu.edu.sg (PWI)

Affiliations Developmental and Biomedical Genetics Laboratory, Institute of Molecular and Cell biology, Agency of Science, Technology and Research (A-STAR), Singapore, Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore
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Guide RNA
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Mutagenesis
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Ribozymes
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Zebrafish
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5' UTR
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Polyacrylamide gel electrophoresis
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