Application of High-Throughput Next-Generation Sequencing for HLA Typing on Buccal Extracted DNA: Results from over 10,000 Donor Recruitment Samples

Yuxin Yin; James H. Lan; David Nguyen; Nicole Valenzuela; Ping Takemura; Yung-Tsi Bolon; Brianna Springer; Katsuyuki Saito; Ying Zheng; Tim Hague; Agnes Pasztor; Gyorgy Horvath; Krisztina Rigo; Elaine F. Reed; Qiuheng Zhang

doi:10.1371/journal.pone.0165810

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Open Access

Peer-reviewed

Research Article

Application of High-Throughput Next-Generation Sequencing for HLA Typing on Buccal Extracted DNA: Results from over 10,000 Donor Recruitment Samples

Yuxin Yin ,

Contributed equally to this work with: Yuxin Yin, James H. Lan

Affiliation UCLA Immunogenetics Center, Department of Pathology & Laboratory Medicine, Los Angeles, CA, United States of America
⨯
James H. Lan ,

Contributed equally to this work with: Yuxin Yin, James H. Lan

Affiliation University of British Columbia Clinician Investigator Program, Vancouver, BC, Canada
⨯
David Nguyen,

Affiliation UCLA Immunogenetics Center, Department of Pathology & Laboratory Medicine, Los Angeles, CA, United States of America
⨯
Nicole Valenzuela,

Affiliation UCLA Immunogenetics Center, Department of Pathology & Laboratory Medicine, Los Angeles, CA, United States of America
⨯
Ping Takemura,

Affiliation UCLA Immunogenetics Center, Department of Pathology & Laboratory Medicine, Los Angeles, CA, United States of America
⨯
Yung-Tsi Bolon,

Affiliation National Marrow Donor Program, Minneapolis, MN, United States of America
⨯
Brianna Springer,

Affiliation National Marrow Donor Program, Minneapolis, MN, United States of America
⨯
Katsuyuki Saito,

Affiliation One Lambda, Thermo Fisher Scientific, Canoga Park, CA, United States of America
⨯
Ying Zheng,

Affiliation UCLA Immunogenetics Center, Department of Pathology & Laboratory Medicine, Los Angeles, CA, United States of America
⨯
Tim Hague,

Affiliation Omixon LTD, Budapest, Hungary
⨯
Agnes Pasztor,

Affiliation Omixon LTD, Budapest, Hungary
⨯
Gyorgy Horvath,

Affiliation Omixon LTD, Budapest, Hungary
⨯
Krisztina Rigo,

Affiliation Omixon LTD, Budapest, Hungary
⨯
Elaine F. Reed,

Affiliation UCLA Immunogenetics Center, Department of Pathology & Laboratory Medicine, Los Angeles, CA, United States of America
⨯
Qiuheng Zhang

* E-mail: jqzhang@mednet.ucla.edu

Affiliation UCLA Immunogenetics Center, Department of Pathology & Laboratory Medicine, Los Angeles, CA, United States of America
⨯

Application of High-Throughput Next-Generation Sequencing for HLA Typing on Buccal Extracted DNA: Results from over 10,000 Donor Recruitment Samples

Yuxin Yin,
James H. Lan,
David Nguyen,
Nicole Valenzuela,
Ping Takemura,
Yung-Tsi Bolon,
Brianna Springer,
Katsuyuki Saito,
Ying Zheng,
Tim Hague

Published: October 31, 2016
https://doi.org/10.1371/journal.pone.0165810

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