16S-23S Internal Transcribed Spacer Region PCR and Sequencer-Based Capillary Gel Electrophoresis has Potential as an Alternative to High Performance Liquid Chromatography for Identification of Slowly Growing Nontuberculous Mycobacteria

Shradha Subedi; Fanrong Kong; Peter Jelfs; Timothy J. Gray; Meng Xiao; Vitali Sintchenko; Sharon C-A Chen

doi:10.1371/journal.pone.0164138

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Peer-reviewed

Research Article

16S-23S Internal Transcribed Spacer Region PCR and Sequencer-Based Capillary Gel Electrophoresis has Potential as an Alternative to High Performance Liquid Chromatography for Identification of Slowly Growing Nontuberculous Mycobacteria

Shradha Subedi ,

* E-mail: subedi_82@yahoo.com

Affiliation Centre for Infectious Diseases and Microbiology Laboratory Services, ICPMR – Pathology West, Westmead Hospital, Westmead, NSW, Australia
⨯
Fanrong Kong,

Affiliation Centre for Infectious Diseases and Microbiology Laboratory Services, ICPMR – Pathology West, Westmead Hospital, Westmead, NSW, Australia
⨯
Peter Jelfs,

Affiliations Centre for Infectious Diseases and Microbiology Laboratory Services, ICPMR – Pathology West, Westmead Hospital, Westmead, NSW, Australia, Centre for Infectious Diseases and Microbiology – Public Health, Westmead Hospital, Westmead, New South Wales, Australia
⨯
Timothy J. Gray,

Affiliation Department of Infectious Diseases and Microbiology, Concord Hospital, Concord, New South Wales, Australia
⨯
Meng Xiao,

Affiliation Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China
⨯
Vitali Sintchenko,

Affiliations Centre for Infectious Diseases and Microbiology Laboratory Services, ICPMR – Pathology West, Westmead Hospital, Westmead, NSW, Australia, Centre for Infectious Diseases and Microbiology – Public Health, Westmead Hospital, Westmead, New South Wales, Australia, Centre for Infectious Diseases and Microbiology, Westmead Hospital and the Marie Bashir Institute for Infectious Diseases and Biosecurity, University of Sydney, Sydney, NSW, Australia
⨯
Sharon C-A Chen

Affiliations Centre for Infectious Diseases and Microbiology Laboratory Services, ICPMR – Pathology West, Westmead Hospital, Westmead, NSW, Australia, Centre for Infectious Diseases and Microbiology – Public Health, Westmead Hospital, Westmead, New South Wales, Australia, Centre for Infectious Diseases and Microbiology, Westmead Hospital and the Marie Bashir Institute for Infectious Diseases and Biosecurity, University of Sydney, Sydney, NSW, Australia
⨯

16S-23S Internal Transcribed Spacer Region PCR and Sequencer-Based Capillary Gel Electrophoresis has Potential as an Alternative to High Performance Liquid Chromatography for Identification of Slowly Growing Nontuberculous Mycobacteria

Shradha Subedi,
Fanrong Kong,
Peter Jelfs,
Timothy J. Gray,
Meng Xiao,
Vitali Sintchenko,
Sharon C-A Chen

Published: October 17, 2016
https://doi.org/10.1371/journal.pone.0164138

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