A Quadruplex Real-Time PCR Assay for the Rapid Detection and Differentiation of the Most Relevant Members of the B. pseudomallei Complex: B. mallei, B. pseudomallei, and B. thailandensis

Chinn-Woan Lowe; Benjamin A. Satterfield; Daniel B. Nelson; Joseph D. Thiriot; Michael J. Heder; Jordon K. March; David S. Drake; Cynthia S. Lew; Annette J. Bunnell; Emily S. Moore; Kim L. O'Neill; Richard A. Robison

doi:10.1371/journal.pone.0164006

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Peer-reviewed

Research Article

A Quadruplex Real-Time PCR Assay for the Rapid Detection and Differentiation of the Most Relevant Members of the B. pseudomallei Complex: B. mallei, B. pseudomallei, and B. thailandensis

Chinn-Woan Lowe,

Affiliation Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, 84602, United States of America
⨯
Benjamin A. Satterfield ,

Contributed equally to this work with: Benjamin A. Satterfield, Daniel B. Nelson

Affiliation Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, 84602, United States of America
⨯
Daniel B. Nelson ,

Contributed equally to this work with: Benjamin A. Satterfield, Daniel B. Nelson

Affiliation Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, 84602, United States of America
⨯
Joseph D. Thiriot,

Affiliation Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, 84602, United States of America
⨯
Michael J. Heder,

Affiliation Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, 84602, United States of America
⨯
Jordon K. March,

Affiliation Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, 84602, United States of America
⨯
David S. Drake,

Affiliation Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, 84602, United States of America
⨯
Cynthia S. Lew,

Affiliation Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, 84602, United States of America
⨯
Annette J. Bunnell,

Affiliation Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, 84602, United States of America
⨯
Emily S. Moore,

Affiliation Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, 84602, United States of America
⨯
Kim L. O'Neill,

Affiliation Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, 84602, United States of America
⨯
Richard A. Robison

* E-mail: richard_robison@byu.edu

Affiliation Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, 84602, United States of America
⨯

A Quadruplex Real-Time PCR Assay for the Rapid Detection and Differentiation of the Most Relevant Members of the B. pseudomallei Complex: B. mallei, B. pseudomallei, and B. thailandensis

Chinn-Woan Lowe,
Benjamin A. Satterfield,
Daniel B. Nelson,
Joseph D. Thiriot,
Michael J. Heder,
Jordon K. March,
David S. Drake,
Cynthia S. Lew,
Annette J. Bunnell,
Emily S. Moore

Published: October 13, 2016
https://doi.org/10.1371/journal.pone.0164006

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Burkholderia mallei
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Polymerase chain reaction
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Burkholderia pseudomallei
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DNA hybridization
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