Scarless Gene Tagging with One-Step Transformation and Two-Step Selection in Saccharomyces cerevisiae and Schizosaccharomyces pombe

Open Access

Peer-reviewed

Research Article

Dirk Landgraf,

Affiliation Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, United States of America

http://orcid.org/0000-0001-9353-4738

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Dann Huh,

Affiliation Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, United States of America
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Erinc Hallacli,

Affiliation Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, United States of America
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Susan Lindquist

* E-mail: lindquist_admin@wi.mit.edu

Affiliations Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, United States of America, Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America
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Saccharomyces cerevisiae
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Polymerase chain reaction
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Schizosaccharomyces pombe
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Yeast
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Cell fusion
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Selection markers
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Plasmid construction
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Genetic loci
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