High-Throughput Analysis of Global DNA Methylation Using Methyl-Sensitive Digestion

Hiromi Shiratori; Carmen Feinweber; Claudia Knothe; Jörn Lötsch; Dominique Thomas; Gerd Geisslinger; Michael J. Parnham; Eduard Resch

doi:10.1371/journal.pone.0163184

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Open Access

Peer-reviewed

Research Article

High-Throughput Analysis of Global DNA Methylation Using Methyl-Sensitive Digestion

Hiromi Shiratori ,

* E-mail: hiromi.shiratori@ime.fraunhofer.de

Affiliation Project Group Translational Medicine and Pharmacology TMP, Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Frankfurt am Main, Germany

http://orcid.org/0000-0002-5826-1401

⨯
Carmen Feinweber,

Affiliation Project Group Translational Medicine and Pharmacology TMP, Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Frankfurt am Main, Germany
⨯
Claudia Knothe,

Affiliation Institute of Clinical Pharmacology, Goethe - University, Frankfurt am Main, Germany
⨯
Jörn Lötsch,

Affiliations Project Group Translational Medicine and Pharmacology TMP, Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Frankfurt am Main, Germany, Institute of Clinical Pharmacology, Goethe - University, Frankfurt am Main, Germany
⨯
Dominique Thomas,

Affiliation Institute of Clinical Pharmacology, Goethe - University, Frankfurt am Main, Germany
⨯
Gerd Geisslinger,

Affiliations Project Group Translational Medicine and Pharmacology TMP, Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Frankfurt am Main, Germany, Institute of Clinical Pharmacology, Goethe - University, Frankfurt am Main, Germany
⨯
Michael J. Parnham,

Affiliation Project Group Translational Medicine and Pharmacology TMP, Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Frankfurt am Main, Germany
⨯
Eduard Resch

Affiliation Project Group Translational Medicine and Pharmacology TMP, Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Frankfurt am Main, Germany
⨯

High-Throughput Analysis of Global DNA Methylation Using Methyl-Sensitive Digestion

Hiromi Shiratori,
Carmen Feinweber,
Claudia Knothe,
Jörn Lötsch,
Dominique Thomas,
Gerd Geisslinger,
Michael J. Parnham,
Eduard Resch

Published: October 17, 2016
https://doi.org/10.1371/journal.pone.0163184

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DNA methylation
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Human genomics
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Methylation
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Fluorescence polarization
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Genomic signal processing
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DNA electrophoresis
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Enzyme assays
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Gel electrophoresis
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