Identification and Characterization of 293T Cell-Derived Exosomes by Profiling the Protein, mRNA and MicroRNA Components

Jing Li; Xiulan Chen; Jiao Yi; Yuchen Liu; Dameng Li; Jifeng Wang; Dongxia Hou; Xiaohong Jiang; Junfeng Zhang; Jin Wang; Ke Zen; Fuquan Yang; Chen-Yu Zhang; Yujing Zhang

doi:10.1371/journal.pone.0163043

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Open Access

Peer-reviewed

Research Article

Identification and Characterization of 293T Cell-Derived Exosomes by Profiling the Protein, mRNA and MicroRNA Components

Jing Li ,

Contributed equally to this work with: Jing Li, Xiulan Chen, Jiao Yi

Affiliation State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute for Life Sciences, School of Life Sciences, Nanjing University, Nanjing, Jiangsu, China
⨯
Xiulan Chen ,

Contributed equally to this work with: Jing Li, Xiulan Chen, Jiao Yi

Affiliation Key Laboratory of Protein and Peptide Pharmaceuticals & Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
⨯
Jiao Yi ,

Contributed equally to this work with: Jing Li, Xiulan Chen, Jiao Yi

Affiliation State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute for Life Sciences, School of Life Sciences, Nanjing University, Nanjing, Jiangsu, China
⨯
Yuchen Liu,

Affiliation State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute for Life Sciences, School of Life Sciences, Nanjing University, Nanjing, Jiangsu, China

http://orcid.org/0000-0001-8922-9976

⨯
Dameng Li,

Affiliation State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute for Life Sciences, School of Life Sciences, Nanjing University, Nanjing, Jiangsu, China
⨯
Jifeng Wang,

Affiliation Key Laboratory of Protein and Peptide Pharmaceuticals & Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
⨯
Dongxia Hou,

Affiliation State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute for Life Sciences, School of Life Sciences, Nanjing University, Nanjing, Jiangsu, China
⨯
Xiaohong Jiang,

Affiliation State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute for Life Sciences, School of Life Sciences, Nanjing University, Nanjing, Jiangsu, China
⨯
Junfeng Zhang,

Affiliation State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute for Life Sciences, School of Life Sciences, Nanjing University, Nanjing, Jiangsu, China
⨯
Jin Wang,

Affiliation State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute for Life Sciences, School of Life Sciences, Nanjing University, Nanjing, Jiangsu, China
⨯
Ke Zen,

Affiliation State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute for Life Sciences, School of Life Sciences, Nanjing University, Nanjing, Jiangsu, China
⨯
Fuquan Yang ,

* E-mail: yjzhang@nju.edu.cn (YZ); cyzhang@nju.edu.cn (CZ); fqyang@sun5.ibp.ac.cn (FY)

Affiliation Key Laboratory of Protein and Peptide Pharmaceuticals & Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
⨯
Chen-Yu Zhang ,

* E-mail: yjzhang@nju.edu.cn (YZ); cyzhang@nju.edu.cn (CZ); fqyang@sun5.ibp.ac.cn (FY)

Affiliation State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute for Life Sciences, School of Life Sciences, Nanjing University, Nanjing, Jiangsu, China
⨯
Yujing Zhang

* E-mail: yjzhang@nju.edu.cn (YZ); cyzhang@nju.edu.cn (CZ); fqyang@sun5.ibp.ac.cn (FY)

Affiliation State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute for Life Sciences, School of Life Sciences, Nanjing University, Nanjing, Jiangsu, China

http://orcid.org/0000-0002-8265-2488

⨯

Identification and Characterization of 293T Cell-Derived Exosomes by Profiling the Protein, mRNA and MicroRNA Components

Jing Li,
Xiulan Chen,
Jiao Yi,
Yuchen Liu,
Dameng Li,
Jifeng Wang,
Dongxia Hou,
Xiaohong Jiang,
Junfeng Zhang,
Jin Wang

Published: September 20, 2016
https://doi.org/10.1371/journal.pone.0163043

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Exosomes
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293T cells
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DNA-binding proteins
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