Cloning, Purification and Characterization of the Collagenase ColA Expressed by Bacillus cereus ATCC 14579

Carmen M. Abfalter; Esther Schönauer; Karthe Ponnuraj; Markus Huemer; Gabriele Gadermaier; Christof Regl; Peter Briza; Fatima Ferreira; Christian G. Huber; Hans Brandstetter; Gernot Posselt; Silja Wessler

doi:10.1371/journal.pone.0162433

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Open Access

Peer-reviewed

Research Article

Cloning, Purification and Characterization of the Collagenase ColA Expressed by Bacillus cereus ATCC 14579

Carmen M. Abfalter,

Affiliation Department of Molecular Biology, Division of Microbiology, Paris-Lodron University of Salzburg, Salzburg, Austria
⨯
Esther Schönauer,

Affiliation Department of Molecular Biology, Division of Structural Biology, Paris-Lodron University of Salzburg, Salzburg, Austria
⨯
Karthe Ponnuraj,

Affiliation Centre of Advanced Study in Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai, India
⨯
Markus Huemer,

Affiliation Department of Molecular Biology, Division of Microbiology, Paris-Lodron University of Salzburg, Salzburg, Austria
⨯
Gabriele Gadermaier,

Affiliation Department of Molecular Biology, Division of Allergy and Immunology, Paris-Lodron University of Salzburg, Salzburg, Austria
⨯
Christof Regl,

Affiliation Department of Molecular Biology, Division of Chemistry and Bioanalytics, Paris-Lodron University of Salzburg, Salzburg, Austria
⨯
Peter Briza,

Affiliation Department of Molecular Biology, Division of Allergy and Immunology, Paris-Lodron University of Salzburg, Salzburg, Austria
⨯
Fatima Ferreira,

Affiliation Department of Molecular Biology, Division of Allergy and Immunology, Paris-Lodron University of Salzburg, Salzburg, Austria
⨯
Christian G. Huber,

Affiliation Department of Molecular Biology, Division of Chemistry and Bioanalytics, Paris-Lodron University of Salzburg, Salzburg, Austria
⨯
Hans Brandstetter,

Affiliation Department of Molecular Biology, Division of Structural Biology, Paris-Lodron University of Salzburg, Salzburg, Austria
⨯
Gernot Posselt,

Affiliation Department of Molecular Biology, Division of Microbiology, Paris-Lodron University of Salzburg, Salzburg, Austria
⨯
Silja Wessler

* E-mail: silja.wessler@sbg.ac.at

Affiliation Department of Molecular Biology, Division of Microbiology, Paris-Lodron University of Salzburg, Salzburg, Austria
⨯

Cloning, Purification and Characterization of the Collagenase ColA Expressed by Bacillus cereus ATCC 14579

Carmen M. Abfalter,
Esther Schönauer,
Karthe Ponnuraj,
Markus Huemer,
Gabriele Gadermaier,
Christof Regl,
Peter Briza,
Fatima Ferreira,
Christian G. Huber,
Hans Brandstetter

Published: September 2, 2016
https://doi.org/10.1371/journal.pone.0162433

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Bacillus cereus
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Collagenases
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Proteases
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Collagens
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Glutathione chromatography
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Bacillus thuringiensis
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