Reconstitution of Protein Translation of Mycobacterium Reveals Functional Conservation and Divergence with the Gram-Negative Bacterium Escherichia coli

Aashish Srivastava; Haruichi Asahara; Meng Zhang; Weijia Zhang; Haiying Liu; Sheng Cui; Qi Jin; Shaorong Chong

doi:10.1371/journal.pone.0162020

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Open Access

Peer-reviewed

Research Article

Reconstitution of Protein Translation of Mycobacterium Reveals Functional Conservation and Divergence with the Gram-Negative Bacterium Escherichia coli

Aashish Srivastava,

Current address: Department of Biochemistry & Biophysics, Texas A&M University, College Station, TX, 77843, United States of America

Affiliation New England Biolabs, Inc., 240 County Road, Ipswich, MA, 01938, United States of America
⨯
Haruichi Asahara,

Affiliation New England Biolabs, Inc., 240 County Road, Ipswich, MA, 01938, United States of America
⨯
Meng Zhang,

Affiliation Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100176, China
⨯
Weijia Zhang,

Affiliation Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100176, China
⨯
Haiying Liu,

Affiliation Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100176, China
⨯
Sheng Cui,

Affiliation Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100176, China
⨯
Qi Jin,

Affiliation Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100176, China
⨯
Shaorong Chong

* E-mail: chong@neb.com

Affiliation New England Biolabs, Inc., 240 County Road, Ipswich, MA, 01938, United States of America
⨯

Reconstitution of Protein Translation of Mycobacterium Reveals Functional Conservation and Divergence with the Gram-Negative Bacterium Escherichia coli

Aashish Srivastava,
Haruichi Asahara,
Meng Zhang,
Weijia Zhang,
Haiying Liu,
Sheng Cui,
Qi Jin,
Shaorong Chong

Published: August 26, 2016
https://doi.org/10.1371/journal.pone.0162020

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Mycobacterium tuberculosis
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Protein translation
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Ribosomes
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Transfer RNA
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Protein synthesis
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Mycobacteria
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Centrifugation
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