Construction of Marker-Free Transgenic Strains of Chlamydomonas reinhardtii Using a Cre/loxP-Mediated Recombinase System

Open Access

Peer-reviewed

Research Article

Yuki Kasai ,

* E-mail: ykasai@kc.chuo-u.ac.jp

Affiliation Department of Biological Sciences, Faculty of Science and Engineering, Chuo University, Bunkyo-ku, Tokyo, Japan
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Shigeaki Harayama

Affiliation Department of Biological Sciences, Faculty of Science and Engineering, Chuo University, Bunkyo-ku, Tokyo, Japan
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Polymerase chain reaction
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Genomics
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Chlamydomonas reinhardtii
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Plasmid construction
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Signal amplification
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Transformation associated recombination
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Marker genes
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Southern blot
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