Environmental DNA Marker Development with Sparse Biological Information: A Case Study on Opossum Shrimp (Mysis diluviana)

Kellie J. Carim; Kyle R. Christianson; Kevin M. McKelvey; William M. Pate; Douglas B. Silver; Brett M. Johnson; Bill T. Galloway; Michael K. Young; Michael K. Schwartz

doi:10.1371/journal.pone.0161664

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Open Access

Peer-reviewed

Research Article

Environmental DNA Marker Development with Sparse Biological Information: A Case Study on Opossum Shrimp (Mysis diluviana)

Kellie J. Carim ,

* E-mail: kelliejcarim@fs.fed.us

Affiliation National Genomics Center for Wildlife and Fish Conservation, U.S. Forest Service Rocky Mountain Research Station, 800 E. Beckwith Ave., Missoula, Montana, 59801, United States of America
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Kyle R. Christianson,

Affiliation Department of Fish, Wildlife and Conservation Biology, Colorado State University, 1474 Campus Delivery, Fort Collins, Colorado, 80523–1474, United States of America
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Kevin M. McKelvey,

Affiliation National Genomics Center for Wildlife and Fish Conservation, U.S. Forest Service Rocky Mountain Research Station, 800 E. Beckwith Ave., Missoula, Montana, 59801, United States of America
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William M. Pate,

Affiliation Department of Fish, Wildlife and Conservation Biology, Colorado State University, 1474 Campus Delivery, Fort Collins, Colorado, 80523–1474, United States of America
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Douglas B. Silver,

Affiliation Department of Fish, Wildlife and Conservation Biology, Colorado State University, 1474 Campus Delivery, Fort Collins, Colorado, 80523–1474, United States of America
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Brett M. Johnson,

Affiliation Department of Fish, Wildlife and Conservation Biology, Colorado State University, 1474 Campus Delivery, Fort Collins, Colorado, 80523–1474, United States of America
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Bill T. Galloway,

Affiliation Department of Fish, Wildlife and Conservation Biology, Colorado State University, 1474 Campus Delivery, Fort Collins, Colorado, 80523–1474, United States of America
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Michael K. Young,

Affiliation National Genomics Center for Wildlife and Fish Conservation, U.S. Forest Service Rocky Mountain Research Station, 800 E. Beckwith Ave., Missoula, Montana, 59801, United States of America
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Michael K. Schwartz

Affiliation National Genomics Center for Wildlife and Fish Conservation, U.S. Forest Service Rocky Mountain Research Station, 800 E. Beckwith Ave., Missoula, Montana, 59801, United States of America
⨯

Environmental DNA Marker Development with Sparse Biological Information: A Case Study on Opossum Shrimp (Mysis diluviana)

Kellie J. Carim,
Kyle R. Christianson,
Kevin M. McKelvey,
William M. Pate,
Douglas B. Silver,
Brett M. Johnson,
Bill T. Galloway,
Michael K. Young,
Michael K. Schwartz

Published: August 23, 2016
https://doi.org/10.1371/journal.pone.0161664

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DNA extraction
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Selection markers
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Polymerase chain reaction
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