One-Tube-Only Standardized Site-Directed Mutagenesis: An Alternative Approach to Generate Amino Acid Substitution Collections

Open Access

Peer-reviewed

Research Article

Janire Mingo,

Affiliation Biomarkers in Cancer Unit, Biocruces Health Research Institute, Barakaldo, Spain
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Asier Erramuzpe,

Affiliation Quantitative Biomedicine Unit, Biocruces Health Research Institute, Barakaldo, Spain
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Sandra Luna,

Affiliation Biomarkers in Cancer Unit, Biocruces Health Research Institute, Barakaldo, Spain
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Olaia Aurtenetxe,

Affiliation Biomarkers in Cancer Unit, Biocruces Health Research Institute, Barakaldo, Spain
⨯
Laura Amo,

Affiliation Biomarkers in Cancer Unit, Biocruces Health Research Institute, Barakaldo, Spain
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Ibai Diez,

Affiliation Quantitative Biomedicine Unit, Biocruces Health Research Institute, Barakaldo, Spain
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Jan T. G. Schepens,

Affiliation Department of Cell Biology, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
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Wiljan J. A. J. Hendriks,

Affiliation Department of Cell Biology, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
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Jesús M. Cortés,

Affiliations Quantitative Biomedicine Unit, Biocruces Health Research Institute, Barakaldo, Spain, IKERBASQUE, Basque Foundation for Science, Bilbao, Spain
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Rafael Pulido

* E-mail: rpulidomurillo@gmail.com; rafael.pulidomurillo@osakidetza.eus

Affiliations Biomarkers in Cancer Unit, Biocruces Health Research Institute, Barakaldo, Spain, IKERBASQUE, Basque Foundation for Science, Bilbao, Spain
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Mutagenesis
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Polymerase chain reaction
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Substitution mutation
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Amino acid substitution
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Plasmid construction
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Mutation
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Nucleotide sequencing
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Mutational analysis
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