In Silico Design and Experimental Validation of siRNAs Targeting Conserved Regions of Multiple Hepatitis C Virus Genotypes

Mahmoud ElHefnawi; TaeKyu Kim; Mona A. Kamar; Saehong Min; Nafisa M. Hassan; Eman El-Ahwany; Heeyoung Kim; Suher Zada; Marwa Amer; Marc P. Windisch

doi:10.1371/journal.pone.0159211

Loading metrics

Open Access

Peer-reviewed

Research Article

In Silico Design and Experimental Validation of siRNAs Targeting Conserved Regions of Multiple Hepatitis C Virus Genotypes

Mahmoud ElHefnawi ,

* E-mail: mahef@aucegypt.edu (MEH); marc.windisch@ip-korea.org (MPW)

Affiliations Informatics and Systems Department, Biomedical Informatics and Chemo-Informatics Group, Centre of Excellence for Advanced Sciences (CEAS), Division of Engineering Research, National Research Centre, Cairo, Egypt, Centre for Informatics, Nile University, Shiekh Zayed City, Egypt, Yousef-Jameel Science and Technology Research Centre, American University in Cairo, New Cairo, Egypt
⨯
TaeKyu Kim,

Affiliation Hepatitis Research Laboratory, Institut Pasteur Korea, 696 Sampyung-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, Republic of Korea
⨯
Mona A. Kamar,

Affiliation Yousef-Jameel Science and Technology Research Centre, American University in Cairo, New Cairo, Egypt
⨯
Saehong Min,

Affiliation Hepatitis Research Laboratory, Institut Pasteur Korea, 696 Sampyung-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, Republic of Korea
⨯
Nafisa M. Hassan,

Affiliation Yousef-Jameel Science and Technology Research Centre, American University in Cairo, New Cairo, Egypt
⨯
Eman El-Ahwany,

Affiliations Biology Department, American University in Cairo, New Cairo, Egypt, Immunology Department, Theodor Bilharz Research Institute, Giza, Egypt
⨯
Heeyoung Kim,

Affiliation Hepatitis Research Laboratory, Institut Pasteur Korea, 696 Sampyung-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, Republic of Korea
⨯
Suher Zada,

Affiliations Yousef-Jameel Science and Technology Research Centre, American University in Cairo, New Cairo, Egypt, Biology Department, American University in Cairo, New Cairo, Egypt
⨯
Marwa Amer,

Affiliations Biology Department, American University in Cairo, New Cairo, Egypt, Faculty of Biotechnology, Misr University for Science and Technology, 6th of October City, Egypt
⨯
Marc P. Windisch

* E-mail: mahef@aucegypt.edu (MEH); marc.windisch@ip-korea.org (MPW)

Affiliation Hepatitis Research Laboratory, Institut Pasteur Korea, 696 Sampyung-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, Republic of Korea
⨯

In Silico Design and Experimental Validation of siRNAs Targeting Conserved Regions of Multiple Hepatitis C Virus Genotypes

Mahmoud ElHefnawi,
TaeKyu Kim,
Mona A. Kamar,
Saehong Min,
Nafisa M. Hassan,
Eman El-Ahwany,
Heeyoung Kim,
Suher Zada,
Marwa Amer,
Marc P. Windisch

Published: July 21, 2016
https://doi.org/10.1371/journal.pone.0159211

Reader Comments (0)

Post a new comment on this article

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

Small interfering RNA
Is the Subject Area "Small interfering RNA" applicable to this article?

Thanks for your feedback.
Viral replication
Is the Subject Area "Viral replication" applicable to this article?

Thanks for your feedback.
Transfection
Is the Subject Area "Transfection" applicable to this article?

Thanks for your feedback.
Hepatitis C virus
Is the Subject Area "Hepatitis C virus" applicable to this article?

Thanks for your feedback.
Internal ribosome entry site
Is the Subject Area "Internal ribosome entry site" applicable to this article?

Thanks for your feedback.
Luciferase
Is the Subject Area "Luciferase" applicable to this article?

Thanks for your feedback.
RNA interference
Is the Subject Area "RNA interference" applicable to this article?

Thanks for your feedback.
Protease inhibitor therapy
Is the Subject Area "Protease inhibitor therapy" applicable to this article?

Thanks for your feedback.