Multiplex Detection of Rare Mutations by Picoliter Droplet Based Digital PCR: Sensitivity and Specificity Considerations

Eleonora Zonta; Fanny Garlan; Nicolas Pécuchet; Karla Perez-Toralla; Ouriel Caen; Coren Milbury; Audrey Didelot; Elizabeth Fabre; Hélène Blons; Pierre Laurent-Puig; Valérie Taly

doi:10.1371/journal.pone.0159094

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Open Access

Peer-reviewed

Research Article

Multiplex Detection of Rare Mutations by Picoliter Droplet Based Digital PCR: Sensitivity and Specificity Considerations

Eleonora Zonta ,

* E-mail: valerie.taly@parisdescartes.fr (VT); eleonora.zonta@parisdescartes.fr (EZ)

Affiliation Université Sorbonne Paris Cité (USPC), INSERM UMR-S1147, CNRS SNC 5014, Centre Universitaire des Saints-Pères, Paris, France
⨯
Fanny Garlan,

Affiliation Université Sorbonne Paris Cité (USPC), INSERM UMR-S1147, CNRS SNC 5014, Centre Universitaire des Saints-Pères, Paris, France
⨯
Nicolas Pécuchet,

Affiliations Université Sorbonne Paris Cité (USPC), INSERM UMR-S1147, CNRS SNC 5014, Centre Universitaire des Saints-Pères, Paris, France, Medical oncology, Hôpital Européen Georges Pompidou (HEGP), Assistance Publique Hôpitaux de Paris (AP-HP), Paris, France
⨯
Karla Perez-Toralla,

Affiliation Université Sorbonne Paris Cité (USPC), INSERM UMR-S1147, CNRS SNC 5014, Centre Universitaire des Saints-Pères, Paris, France
⨯
Ouriel Caen,

Affiliation Université Sorbonne Paris Cité (USPC), INSERM UMR-S1147, CNRS SNC 5014, Centre Universitaire des Saints-Pères, Paris, France
⨯
Coren Milbury,

Affiliation RainDance Technologies, Billerica, Massachusetts, United States of America
⨯
Audrey Didelot,

Affiliation Université Sorbonne Paris Cité (USPC), INSERM UMR-S1147, CNRS SNC 5014, Centre Universitaire des Saints-Pères, Paris, France
⨯
Elizabeth Fabre,

Affiliations Université Sorbonne Paris Cité (USPC), INSERM UMR-S1147, CNRS SNC 5014, Centre Universitaire des Saints-Pères, Paris, France, Medical oncology, Hôpital Européen Georges Pompidou (HEGP), Assistance Publique Hôpitaux de Paris (AP-HP), Paris, France
⨯
Hélène Blons,

Affiliations Université Sorbonne Paris Cité (USPC), INSERM UMR-S1147, CNRS SNC 5014, Centre Universitaire des Saints-Pères, Paris, France, Department of Biochemistry, Unit of pharmacogenetic and molecular oncology, Hôpital Européen Georges Pompidou (HEGP), Assistance Publique Hôpitaux de Paris (AP-HP), Paris, France
⨯
Pierre Laurent-Puig,

Affiliations Université Sorbonne Paris Cité (USPC), INSERM UMR-S1147, CNRS SNC 5014, Centre Universitaire des Saints-Pères, Paris, France, Department of Biochemistry, Unit of pharmacogenetic and molecular oncology, Hôpital Européen Georges Pompidou (HEGP), Assistance Publique Hôpitaux de Paris (AP-HP), Paris, France
⨯
Valérie Taly

* E-mail: valerie.taly@parisdescartes.fr (VT); eleonora.zonta@parisdescartes.fr (EZ)

Affiliation Université Sorbonne Paris Cité (USPC), INSERM UMR-S1147, CNRS SNC 5014, Centre Universitaire des Saints-Pères, Paris, France
⨯

Multiplex Detection of Rare Mutations by Picoliter Droplet Based Digital PCR: Sensitivity and Specificity Considerations

Eleonora Zonta,
Fanny Garlan,
Nicolas Pécuchet,
Karla Perez-Toralla,
Ouriel Caen,
Coren Milbury,
Audrey Didelot,
Elizabeth Fabre,
Hélène Blons,
Pierre Laurent-Puig

Published: July 14, 2016
https://doi.org/10.1371/journal.pone.0159094

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