Promoter Screening from Bacillus subtilis in Various Conditions Hunting for Synthetic Biology and Industrial Applications

Yafeng Song; Jonas M. Nikoloff; Gang Fu; Jingqi Chen; Qinggang Li; Nengzhong Xie; Ping Zheng; Jibin Sun; Dawei Zhang

doi:10.1371/journal.pone.0158447

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Open Access

Peer-reviewed

Research Article

Promoter Screening from Bacillus subtilis in Various Conditions Hunting for Synthetic Biology and Industrial Applications

Yafeng Song ,

Contributed equally to this work with: Yafeng Song, Jonas M. Nikoloff

Affiliations Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, P. R. China, Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, P. R. China
⨯
Jonas M. Nikoloff ,

Contributed equally to this work with: Yafeng Song, Jonas M. Nikoloff

Affiliation Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, P. R. China
⨯
Gang Fu,

Affiliations Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, P. R. China, Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, P. R. China
⨯
Jingqi Chen,

Affiliations Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, P. R. China, Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, P. R. China
⨯
Qinggang Li,

Affiliations Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, P. R. China, Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, P. R. China
⨯
Nengzhong Xie,

Affiliations National Engineering Research Center for Non-food Biorefinery, State Key Laboratory of Non-food Biomass Energy and Enzyme Technology, Nanning 5300074, P. R. China, Guangxi Biomass Industrialization Engineering Institute, Guangxi Academy of Sciences, Nanning 530007, P. R. China
⨯
Ping Zheng,

Affiliations Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, P. R. China, Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, P. R. China
⨯
Jibin Sun,

Affiliations Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, P. R. China, Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, P. R. China
⨯
Dawei Zhang

* E-mail: zhang_dw@tib.cas.cn

Affiliations Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, P. R. China, Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, P. R. China
⨯

Promoter Screening from Bacillus subtilis in Various Conditions Hunting for Synthetic Biology and Industrial Applications

Yafeng Song,
Jonas M. Nikoloff,
Gang Fu,
Jingqi Chen,
Qinggang Li,
Nengzhong Xie,
Ping Zheng,
Jibin Sun,
Dawei Zhang

Published: July 5, 2016
https://doi.org/10.1371/journal.pone.0158447

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Bacillus subtilis
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Fluorescence-activated cell sorting
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Ethanol
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Heat shock response
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Plasmid construction
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Polymerase chain reaction
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Protein expression
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