Differentiation/Purification Protocol for Retinal Pigment Epithelium from Mouse Induced Pluripotent Stem Cells as a Research Tool

Yuko Iwasaki; Sunao Sugita; Michiko Mandai; Shigenobu Yonemura; Akishi Onishi; Shin-ichiro Ito; Manabu Mochizuki; Kyoko Ohno-Matsui; Masayo Takahashi

doi:10.1371/journal.pone.0158282

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Peer-reviewed

Research Article

Differentiation/Purification Protocol for Retinal Pigment Epithelium from Mouse Induced Pluripotent Stem Cells as a Research Tool

Yuko Iwasaki,

Affiliations Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, Kobe, Hyogo, Japan, Department of Ophthalmology & Visual Science, Graduate School of Medical and Dental Science, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan
⨯
Sunao Sugita,

Affiliation Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, Kobe, Hyogo, Japan
⨯
Michiko Mandai,

Affiliation Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, Kobe, Hyogo, Japan
⨯
Shigenobu Yonemura,

Affiliations Ultrastructural Research Team, RIKEN Center for Life Science Technologies, Kobe, Hyogo, Japan, Department of Cell Biology, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan
⨯
Akishi Onishi,

Affiliation Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, Kobe, Hyogo, Japan
⨯
Shin-ichiro Ito,

Affiliation Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, Kobe, Hyogo, Japan
⨯
Manabu Mochizuki,

Affiliation Department of Ophthalmology & Visual Science, Graduate School of Medical and Dental Science, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan
⨯
Kyoko Ohno-Matsui,

Affiliation Department of Ophthalmology & Visual Science, Graduate School of Medical and Dental Science, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan
⨯
Masayo Takahashi

* E-mail: retinalab@cdb.riken.jp

Affiliation Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, Kobe, Hyogo, Japan
⨯

Differentiation/Purification Protocol for Retinal Pigment Epithelium from Mouse Induced Pluripotent Stem Cells as a Research Tool

Yuko Iwasaki,
Sunao Sugita,
Michiko Mandai,
Shigenobu Yonemura,
Akishi Onishi,
Shin-ichiro Ito,
Manabu Mochizuki,
Kyoko Ohno-Matsui,
Masayo Takahashi

Published: July 6, 2016
https://doi.org/10.1371/journal.pone.0158282

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