Novel Clostridium difficile Anti-Toxin (TcdA and TcdB) Humanized Monoclonal Antibodies Demonstrate In Vitro Neutralization across a Broad Spectrum of Clinical Strains and In Vivo Potency in a Hamster Spore Challenge Model

Hongyu Qiu; Robyn Cassan; Darrell Johnstone; Xiaobing Han; Antony George Joyee; Monica McQuoid; Andrea Masi; John Merluza; Bryce Hrehorak; Ross Reid; Kieron Kennedy; Bonnie Tighe; Carla Rak; Melanie Leonhardt; Brian Dupas; Laura Saward; Jody D. Berry; Cory L. Nykiforuk

doi:10.1371/journal.pone.0157970

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Peer-reviewed

Research Article

Novel Clostridium difficile Anti-Toxin (TcdA and TcdB) Humanized Monoclonal Antibodies Demonstrate In Vitro Neutralization across a Broad Spectrum of Clinical Strains and In Vivo Potency in a Hamster Spore Challenge Model

Hongyu Qiu,

Affiliation Cangene Corporation, a subsidiary of Emergent BioSolutions Inc., 155 Innovation Drive, Winnipeg, MB, R3T 5Y3, Canada
⨯
Robyn Cassan,

Affiliation Cangene Corporation, a subsidiary of Emergent BioSolutions Inc., 155 Innovation Drive, Winnipeg, MB, R3T 5Y3, Canada
⨯
Darrell Johnstone,

Affiliation Cangene Corporation, a subsidiary of Emergent BioSolutions Inc., 155 Innovation Drive, Winnipeg, MB, R3T 5Y3, Canada
⨯
Xiaobing Han,

Affiliation Cangene Corporation, a subsidiary of Emergent BioSolutions Inc., 155 Innovation Drive, Winnipeg, MB, R3T 5Y3, Canada
⨯
Antony George Joyee,

Affiliation Cangene Corporation, a subsidiary of Emergent BioSolutions Inc., 155 Innovation Drive, Winnipeg, MB, R3T 5Y3, Canada
⨯
Monica McQuoid,

Affiliation Cangene Corporation, a subsidiary of Emergent BioSolutions Inc., 155 Innovation Drive, Winnipeg, MB, R3T 5Y3, Canada
⨯
Andrea Masi,

Affiliation Cangene Corporation, a subsidiary of Emergent BioSolutions Inc., 155 Innovation Drive, Winnipeg, MB, R3T 5Y3, Canada
⨯
John Merluza,

Affiliation Cangene Corporation, a subsidiary of Emergent BioSolutions Inc., 155 Innovation Drive, Winnipeg, MB, R3T 5Y3, Canada
⨯
Bryce Hrehorak,

Affiliation Cangene Corporation, a subsidiary of Emergent BioSolutions Inc., 155 Innovation Drive, Winnipeg, MB, R3T 5Y3, Canada
⨯
Ross Reid,

Affiliation Cangene Corporation, a subsidiary of Emergent BioSolutions Inc., 155 Innovation Drive, Winnipeg, MB, R3T 5Y3, Canada
⨯
Kieron Kennedy,

Affiliation Cangene Corporation, a subsidiary of Emergent BioSolutions Inc., 155 Innovation Drive, Winnipeg, MB, R3T 5Y3, Canada
⨯
Bonnie Tighe,

Affiliation Cangene Corporation, a subsidiary of Emergent BioSolutions Inc., 155 Innovation Drive, Winnipeg, MB, R3T 5Y3, Canada
⨯
Carla Rak,

Affiliation Cangene Corporation, a subsidiary of Emergent BioSolutions Inc., 155 Innovation Drive, Winnipeg, MB, R3T 5Y3, Canada
⨯
Melanie Leonhardt,

Affiliation Cangene Corporation, a subsidiary of Emergent BioSolutions Inc., 155 Innovation Drive, Winnipeg, MB, R3T 5Y3, Canada
⨯
Brian Dupas,

Affiliation Cangene Corporation, a subsidiary of Emergent BioSolutions Inc., 155 Innovation Drive, Winnipeg, MB, R3T 5Y3, Canada
⨯
Laura Saward,

Affiliation Cangene Corporation, a subsidiary of Emergent BioSolutions Inc., 155 Innovation Drive, Winnipeg, MB, R3T 5Y3, Canada
⨯
Jody D. Berry,

Current Address: Grifols, Emeryville, CA, 94608, United States of America

Affiliation Cangene Corporation, a subsidiary of Emergent BioSolutions Inc., 155 Innovation Drive, Winnipeg, MB, R3T 5Y3, Canada
⨯
[ ... ],
Cory L. Nykiforuk

* E-mail: cnykiforuk@ebsi.com

Affiliation Cangene Corporation, a subsidiary of Emergent BioSolutions Inc., 155 Innovation Drive, Winnipeg, MB, R3T 5Y3, Canada
⨯
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Novel Clostridium difficile Anti-Toxin (TcdA and TcdB) Humanized Monoclonal Antibodies Demonstrate In Vitro Neutralization across a Broad Spectrum of Clinical Strains and In Vivo Potency in a Hamster Spore Challenge Model

Hongyu Qiu,
Robyn Cassan,
Darrell Johnstone,
Xiaobing Han,
Antony George Joyee,
Monica McQuoid,
Andrea Masi,
John Merluza,
Bryce Hrehorak,
Ross Reid

Published: June 23, 2016
https://doi.org/10.1371/journal.pone.0157970

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