High Level Expression and Purification of Recombinant Proteins from Escherichia coli with AK-TAG

Open Access

Peer-reviewed

Research Article

Dan Luo,

Affiliation Department of Biochemistry & Molecular Biology, School of Life Sciences, Sichuan University, Chengdu, China
⨯
Caixia Wen,

Affiliation Department of Biochemistry & Molecular Biology, School of Life Sciences, Sichuan University, Chengdu, China
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Rongchuan Zhao,

Affiliation Department of Biochemistry & Molecular Biology, School of Life Sciences, Sichuan University, Chengdu, China
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Xinyu Liu,

Affiliation Department of Biochemistry & Molecular Biology, School of Life Sciences, Sichuan University, Chengdu, China
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Xinxin Liu,

Affiliation Department of Biochemistry & Molecular Biology, School of Life Sciences, Sichuan University, Chengdu, China
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Jingjing Cui,

Affiliation Department of Biochemistry & Molecular Biology, School of Life Sciences, Sichuan University, Chengdu, China
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Joshua G. Liang,

Affiliation Clover Biopharmaceuticals, Chengdu, China
⨯
Peng Liang

* E-mail: liangpeng@scu.edu.cn; liang.peng@cloverbiopharma.com

Affiliations Department of Biochemistry & Molecular Biology, School of Life Sciences, Sichuan University, Chengdu, China, Clover Biopharmaceuticals, Chengdu, China, GenHunter Corporation, Grassmere Park, Nashville, United States of America
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Recombinant proteins
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Elution
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Thrombin
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Cell fusion
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Protein expression
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DNA-binding proteins
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Ligases
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Vector cloning
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