The Commercial Antibodies Widely Used to Measure H3 K56 Acetylation Are Non-Specific in Human and Drosophila Cells

Sangita Pal; Hillary Graves; Ryosuke Ohsawa; Ting-hsiang Huang; Pingping Wang; Laura Harmacek; Jessica Tyler

doi:10.1371/journal.pone.0155409

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Peer-reviewed

Research Article

The Commercial Antibodies Widely Used to Measure H3 K56 Acetylation Are Non-Specific in Human and Drosophila Cells

Sangita Pal ,

¶‡ Equal first authors

Affiliations Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, 1300 York Avenue, New York, New York, 10065, United States of America, Department of Epigenetics and Molecular Carcinogenesis, University of Texas MD Anderson Cancer Center, Houston, Texas, 77030, United States of America
⨯
Hillary Graves ,

¶‡ Equal first authors

Affiliation Department of Epigenetics and Molecular Carcinogenesis, University of Texas MD Anderson Cancer Center, Houston, Texas, 77030, United States of America
⨯
Ryosuke Ohsawa ,

¶‡ Equal first authors

Affiliation Department of Epigenetics and Molecular Carcinogenesis, University of Texas MD Anderson Cancer Center, Houston, Texas, 77030, United States of America
⨯
Ting-hsiang Huang,

Affiliations Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, 1300 York Avenue, New York, New York, 10065, United States of America, Department of Epigenetics and Molecular Carcinogenesis, University of Texas MD Anderson Cancer Center, Houston, Texas, 77030, United States of America
⨯
Pingping Wang,

Affiliations Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, 1300 York Avenue, New York, New York, 10065, United States of America, Department of Epigenetics and Molecular Carcinogenesis, University of Texas MD Anderson Cancer Center, Houston, Texas, 77030, United States of America
⨯
Laura Harmacek,

Affiliation Department of Cell Biology, University of Colorado School of Medicine, Aurora, Colorado, United States of America
⨯
Jessica Tyler

* E-mail: jet2021@med.cornell.edu

Affiliations Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, 1300 York Avenue, New York, New York, 10065, United States of America, Department of Epigenetics and Molecular Carcinogenesis, University of Texas MD Anderson Cancer Center, Houston, Texas, 77030, United States of America
⨯

The Commercial Antibodies Widely Used to Measure H3 K56 Acetylation Are Non-Specific in Human and Drosophila Cells

Sangita Pal,
Hillary Graves,
Ryosuke Ohsawa,
Ting-hsiang Huang,
Pingping Wang,
Laura Harmacek,
Jessica Tyler

Published: May 17, 2016
https://doi.org/10.1371/journal.pone.0155409

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Histones
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Immunohistochemistry techniques
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Acetylation
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