Overcoming the Refractory Expression of Secreted Recombinant Proteins in Mammalian Cells through Modification of the Signal Peptide and Adjacent Amino Acids

Open Access

Peer-reviewed

Research Article

Gülin Güler-Gane,

Affiliation Department of Antibody Discovery and Protein Engineering, MedImmune Ltd., Granta Park, Cambridge, United Kingdom
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Sara Kidd,

Affiliation Department of Antibody Discovery and Protein Engineering, MedImmune Ltd., Granta Park, Cambridge, United Kingdom
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Sudharsan Sridharan,

Affiliation Department of Antibody Discovery and Protein Engineering, MedImmune Ltd., Granta Park, Cambridge, United Kingdom
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Tristan J. Vaughan,

Affiliation Department of Antibody Discovery and Protein Engineering, MedImmune Ltd., Granta Park, Cambridge, United Kingdom
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Trevor C. I. Wilkinson,

Affiliation Department of Antibody Discovery and Protein Engineering, MedImmune Ltd., Granta Park, Cambridge, United Kingdom
⨯
Natalie J. Tigue

* E-mail: tiguen@medimmune.com

Affiliation Department of Antibody Discovery and Protein Engineering, MedImmune Ltd., Granta Park, Cambridge, United Kingdom

http://orcid.org/0000-0001-9916-0255

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Signal peptides
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Alanine
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Recombinant proteins
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Protein sequencing
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Protein expression
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Proline
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Protein secretion
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Transfection
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