Optimization of Liver Decellularization Maintains Extracellular Matrix Micro-Architecture and Composition Predisposing to Effective Cell Seeding

Panagiotis Maghsoudlou; Fanourios Georgiades; Holly Smith; Anna Milan; Panicos Shangaris; Luca Urbani; Stavros P. Loukogeorgakis; Benedetta Lombardi; Giuseppe Mazza; Charlotte Hagen; Neil J. Sebire; Mark Turmaine; Simon Eaton; Alessandro Olivo; Jasminka Godovac-Zimmermann; Massimo Pinzani; Paul Gissen; Paolo De Coppi

doi:10.1371/journal.pone.0155324

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Peer-reviewed

Research Article

Optimization of Liver Decellularization Maintains Extracellular Matrix Micro-Architecture and Composition Predisposing to Effective Cell Seeding

Panagiotis Maghsoudlou,

Affiliation Department of Stem Cells and Regenerative Medicine, UCL Institute of Child Health and Great Ormond Street Hospital, 30 Guilford Street, London, WC1N 1EH, United Kingdom
⨯
Fanourios Georgiades,

Affiliation Department of Stem Cells and Regenerative Medicine, UCL Institute of Child Health and Great Ormond Street Hospital, 30 Guilford Street, London, WC1N 1EH, United Kingdom
⨯
Holly Smith,

Affiliation MRC Laboratory for Molecular Cell Biology, University College London, London, WC1E 6BT, United Kingdom
⨯
Anna Milan,

Affiliation Department of Stem Cells and Regenerative Medicine, UCL Institute of Child Health and Great Ormond Street Hospital, 30 Guilford Street, London, WC1N 1EH, United Kingdom
⨯
Panicos Shangaris,

Affiliation Department of Stem Cells and Regenerative Medicine, UCL Institute of Child Health and Great Ormond Street Hospital, 30 Guilford Street, London, WC1N 1EH, United Kingdom
⨯
Luca Urbani,

Affiliation Department of Stem Cells and Regenerative Medicine, UCL Institute of Child Health and Great Ormond Street Hospital, 30 Guilford Street, London, WC1N 1EH, United Kingdom
⨯
Stavros P. Loukogeorgakis,

Affiliation Department of Stem Cells and Regenerative Medicine, UCL Institute of Child Health and Great Ormond Street Hospital, 30 Guilford Street, London, WC1N 1EH, United Kingdom
⨯
Benedetta Lombardi,

Affiliation London Center for Nephrology, University College London, London, WC1N 1EH, United Kingdom
⨯
Giuseppe Mazza,

Affiliation Institute for Liver and Digestive Health, University College London, London, WC1N 1EH, United Kingdom
⨯
Charlotte Hagen,

Affiliation Department of Medical Physics & Bioengineering, University College London, London, WC1N 1EH, United Kingdom
⨯
Neil J. Sebire,

Affiliation Department of Histopathology, UCL Institute of Child Health and Great Ormond Street Hospital, London, WC1N 1EH, United Kingdom
⨯
Mark Turmaine,

Affiliation Division of Bioscience, University College London, London, WC1N 1EH, United Kingdom
⨯
Simon Eaton,

Affiliation Department of Stem Cells and Regenerative Medicine, UCL Institute of Child Health and Great Ormond Street Hospital, 30 Guilford Street, London, WC1N 1EH, United Kingdom
⨯
Alessandro Olivo,

Affiliation Department of Medical Physics & Bioengineering, University College London, London, WC1N 1EH, United Kingdom
⨯
Jasminka Godovac-Zimmermann,

Affiliation London Center for Nephrology, University College London, London, WC1N 1EH, United Kingdom
⨯
Massimo Pinzani,

Affiliation Institute for Liver and Digestive Health, University College London, London, WC1N 1EH, United Kingdom
⨯
Paul Gissen,

Affiliation MRC Laboratory for Molecular Cell Biology, University College London, London, WC1E 6BT, United Kingdom
⨯
[ ... ],
Paolo De Coppi

* E-mail: p.decoppi@ucl.ac.uk

Affiliation Department of Stem Cells and Regenerative Medicine, UCL Institute of Child Health and Great Ormond Street Hospital, 30 Guilford Street, London, WC1N 1EH, United Kingdom
⨯
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Optimization of Liver Decellularization Maintains Extracellular Matrix Micro-Architecture and Composition Predisposing to Effective Cell Seeding

Panagiotis Maghsoudlou,
Fanourios Georgiades,
Holly Smith,
Anna Milan,
Panicos Shangaris,
Luca Urbani,
Stavros P. Loukogeorgakis,
Benedetta Lombardi,
Giuseppe Mazza,
Charlotte Hagen

Published: May 9, 2016
https://doi.org/10.1371/journal.pone.0155324

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