Enhanced snoMEN Vectors Facilitate Establishment of GFP–HIF-1α Protein Replacement Human Cell Lines

Motoharu Ono; Kayo Yamada; Dalila Bensaddek; Vackar Afzal; John Biddlestone; Brian Ortmann; Sharon Mudie; Vincent Boivin; Michelle S. Scott; Sonia Rocha; Angus I. Lamond

doi:10.1371/journal.pone.0154759

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Open Access

Peer-reviewed

Research Article

Enhanced snoMEN Vectors Facilitate Establishment of GFP–HIF-1α Protein Replacement Human Cell Lines

Motoharu Ono ,

Contributed equally to this work with: Motoharu Ono, Kayo Yamada

Affiliation Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee, United Kingdom
⨯
Kayo Yamada ,

Contributed equally to this work with: Motoharu Ono, Kayo Yamada

Affiliation Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee, United Kingdom
⨯
Dalila Bensaddek,

Affiliation Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee, United Kingdom
⨯
Vackar Afzal,

Affiliation Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee, United Kingdom
⨯
John Biddlestone,

Affiliation Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee, United Kingdom
⨯
Brian Ortmann,

Affiliation Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee, United Kingdom
⨯
Sharon Mudie,

Affiliation Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee, United Kingdom
⨯
Vincent Boivin,

Affiliation Department of Biochemistry and RNA Group, Faculty of Medicine and Health Sciences, University of Sherbrooke, Sherbrooke, Canada
⨯
Michelle S. Scott,

Affiliation Department of Biochemistry and RNA Group, Faculty of Medicine and Health Sciences, University of Sherbrooke, Sherbrooke, Canada
⨯
Sonia Rocha,

Affiliation Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee, United Kingdom
⨯
Angus I. Lamond

* E-mail: a.i.lamond@dundee.ac.uk

Affiliation Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee, United Kingdom
⨯

Enhanced snoMEN Vectors Facilitate Establishment of GFP–HIF-1α Protein Replacement Human Cell Lines

Motoharu Ono,
Kayo Yamada,
Dalila Bensaddek,
Vackar Afzal,
John Biddlestone,
Brian Ortmann,
Sharon Mudie,
Vincent Boivin,
Michelle S. Scott,
Sonia Rocha

Published: April 29, 2016
https://doi.org/10.1371/journal.pone.0154759

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Small nucleolar RNA
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Transfection
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Plasmid construction
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HeLa cells
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Gene expression
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Hypoxia
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Vector construction
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Protein expression
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