Comparison of Boiling and Robotics Automation Method in DNA Extraction for Metagenomic Sequencing of Human Oral Microbes

• Junya Yamagishi ,

  Contributed equally to this work with: Junya Yamagishi, Yukuto Sato

  Affiliations Division of Collaboration and Education, Research Center for Zoonosis Control, Hokkaido University, North 20, West 10 Kita-ku, Sapporo, Hokkaido, 001–0020, Japan, Global Station for Zoonosis Control, GI-CoRE, Hokkaido University, North 20, West 10 Kita-ku, Sapporo, Hokkaido, 001–0020, Japan, Department of Integrative Genomics, Tohoku Medical Megabank Organization, Tohoku University, 2–1, Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980–8573, Japan

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• Yukuto Sato ,

  Contributed equally to this work with: Junya Yamagishi, Yukuto Sato

  Affiliation Department of Integrative Genomics, Tohoku Medical Megabank Organization, Tohoku University, 2–1, Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980–8573, Japan

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• Natsuko Shinozaki,

  Affiliation Department of Integrative Genomics, Tohoku Medical Megabank Organization, Tohoku University, 2–1, Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980–8573, Japan

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• Bin Ye,

  Affiliation Department of Integrative Genomics, Tohoku Medical Megabank Organization, Tohoku University, 2–1, Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980–8573, Japan

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• Akito Tsuboi,

  Affiliation Department of Community Medical Supports, Tohoku Medical Megabank Organization, Tohoku University, 2–1, Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980–8573, Japan

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• Masao Nagasaki ,

  * E-mail: nagasaki@megabank.tohoku.ac.jp (MN); ryamasi@megabank.tohoku.ac.jp (RY)

  Affiliation Department of Integrative Genomics, Tohoku Medical Megabank Organization, Tohoku University, 2–1, Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980–8573, Japan

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• Riu Yamashita

  * E-mail: nagasaki@megabank.tohoku.ac.jp (MN); ryamasi@megabank.tohoku.ac.jp (RY)

  Affiliation Department of Integrative Genomics, Tohoku Medical Megabank Organization, Tohoku University, 2–1, Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980–8573, Japan

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