Production of Functionally Active and Immunogenic Non-Glycosylated Protective Antigen from Bacillus anthracis in Nicotiana benthamiana by Co-Expression with Peptide-N-Glycosidase F (PNGase F) of Flavobacterium meningosepticum

Tarlan Mamedov; Jessica A. Chichester; R. Mark Jones; Ananya Ghosh; Megan V. Coffin; Kristina Herschbach; Alexey I. Prokhnevsky; Stephen J. Streatfield; Vidadi Yusibov

doi:10.1371/journal.pone.0153956

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Peer-reviewed

Research Article

Production of Functionally Active and Immunogenic Non-Glycosylated Protective Antigen from Bacillus anthracis in Nicotiana benthamiana by Co-Expression with Peptide-N-Glycosidase F (PNGase F) of Flavobacterium meningosepticum

Tarlan Mamedov ,

* E-mail: tmammedov@gmail.com (TM); Vidadi.Yusibov@fhcmb.org (VY)

Current Address: Department of Agricultural Biotechnology, Akdeniz University, Antalya 07985, Turkey

Affiliation Fraunhofer USA Center for Molecular Biotechnology, Newark, Delaware, United States of America
⨯
Jessica A. Chichester,

Affiliation Fraunhofer USA Center for Molecular Biotechnology, Newark, Delaware, United States of America
⨯
R. Mark Jones,

Affiliation Fraunhofer USA Center for Molecular Biotechnology, Newark, Delaware, United States of America
⨯
Ananya Ghosh,

Current Address: Siemens Healthcare, 500 Gbc Drive, Newark, Delaware 19702, United States of America

Affiliation Fraunhofer USA Center for Molecular Biotechnology, Newark, Delaware, United States of America
⨯
Megan V. Coffin,

Affiliation Fraunhofer USA Center for Molecular Biotechnology, Newark, Delaware, United States of America
⨯
Kristina Herschbach,

Current Address: Merck Millipore, Frankfurter Straße 250, 64293 Darmstadt, Germany

Affiliation Fraunhofer USA Center for Molecular Biotechnology, Newark, Delaware, United States of America
⨯
Alexey I. Prokhnevsky,

Affiliation Fraunhofer USA Center for Molecular Biotechnology, Newark, Delaware, United States of America
⨯
Stephen J. Streatfield,

Affiliation Fraunhofer USA Center for Molecular Biotechnology, Newark, Delaware, United States of America
⨯
Vidadi Yusibov

* E-mail: tmammedov@gmail.com (TM); Vidadi.Yusibov@fhcmb.org (VY)

Affiliation Fraunhofer USA Center for Molecular Biotechnology, Newark, Delaware, United States of America
⨯

Production of Functionally Active and Immunogenic Non-Glycosylated Protective Antigen from Bacillus anthracis in Nicotiana benthamiana by Co-Expression with Peptide-N-Glycosidase F (PNGase F) of Flavobacterium meningosepticum

Tarlan Mamedov,
Jessica A. Chichester,
R. Mark Jones,
Ananya Ghosh,
Megan V. Coffin,
Kristina Herschbach,
Alexey I. Prokhnevsky,
Stephen J. Streatfield,
Vidadi Yusibov

Published: April 21, 2016
https://doi.org/10.1371/journal.pone.0153956

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