Activity of the Human Rhinovirus 3C Protease Studied in Various Buffers, Additives and Detergents Solutions for Recombinant Protein Production

Raheem Ullah; Majid Ali Shah; Soban Tufail; Fouzia Ismat; Muhammad Imran; Mazhar Iqbal; Osman Mirza; Moazur Rhaman

doi:10.1371/journal.pone.0153436

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Open Access

Peer-reviewed

Research Article

Activity of the Human Rhinovirus 3C Protease Studied in Various Buffers, Additives and Detergents Solutions for Recombinant Protein Production

Raheem Ullah ,

Contributed equally to this work with: Raheem Ullah, Majid Ali Shah

Affiliations Drug Discovery and Structural Biology group, Health Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan, Pakistan Institute of Engineering and Applied Sciences, P.O. Nilore, Islamabad, Pakistan
⨯
Majid Ali Shah ,

Contributed equally to this work with: Raheem Ullah, Majid Ali Shah

Affiliations Drug Discovery and Structural Biology group, Health Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan, Pakistan Institute of Engineering and Applied Sciences, P.O. Nilore, Islamabad, Pakistan
⨯
Soban Tufail,

Affiliation Drug Discovery and Structural Biology group, Health Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan
⨯
Fouzia Ismat,

Affiliation Drug Discovery and Structural Biology group, Health Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan
⨯
Muhammad Imran,

Current address: Forman Christian College (A Chartered University), Lahore, Pakistan

Affiliation Drug Discovery and Structural Biology group, Health Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan
⨯
Mazhar Iqbal,

Affiliations Drug Discovery and Structural Biology group, Health Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan, Pakistan Institute of Engineering and Applied Sciences, P.O. Nilore, Islamabad, Pakistan
⨯
Osman Mirza,

Affiliation Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
⨯
Moazur Rhaman

* E-mail: moazur.rahman@fulbrightmail.org; moaz@nibge.org

Affiliations Drug Discovery and Structural Biology group, Health Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan, Pakistan Institute of Engineering and Applied Sciences, P.O. Nilore, Islamabad, Pakistan
⨯

Activity of the Human Rhinovirus 3C Protease Studied in Various Buffers, Additives and Detergents Solutions for Recombinant Protein Production

Raheem Ullah,
Majid Ali Shah,
Soban Tufail,
Fouzia Ismat,
Muhammad Imran,
Mazhar Iqbal,
Osman Mirza,
Moazur Rhaman

Published: April 19, 2016
https://doi.org/10.1371/journal.pone.0153436

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04 May 2016
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