Unstable Expression of Commonly Used Reference Genes in Rat Pancreatic Islets Early after Isolation Affects Results of Gene Expression Studies

Lucie Kosinová; Monika Cahová; Eva Fábryová; Irena Týcová; Tomáš Koblas; Ivan Leontovyč; František Saudek; Jan Kříž

doi:10.1371/journal.pone.0152664

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Peer-reviewed

Research Article

Unstable Expression of Commonly Used Reference Genes in Rat Pancreatic Islets Early after Isolation Affects Results of Gene Expression Studies

Lucie Kosinová ,

* E-mail: kosl@ikem.cz (LK); jkri@ikem.cz (JK)

Affiliations Laboratory of Pancreatic Islets, Center of Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic
⨯
Monika Cahová,

Affiliation Department of Metabolism and Diabetes, Center of Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
⨯
Eva Fábryová,

Affiliation Laboratory of Pancreatic Islets, Center of Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
⨯
Irena Týcová,

Affiliation Transplant Laboratory, Center of Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
⨯
Tomáš Koblas,

Affiliation Laboratory of Pancreatic Islets, Center of Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
⨯
Ivan Leontovyč,

Affiliation Laboratory of Pancreatic Islets, Center of Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
⨯
František Saudek,

Affiliations Laboratory of Pancreatic Islets, Center of Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic, Department of Diabetes, Center of Diabetes, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
⨯
Jan Kříž

* E-mail: kosl@ikem.cz (LK); jkri@ikem.cz (JK)

Affiliation Department of Diabetes, Center of Diabetes, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
⨯

Unstable Expression of Commonly Used Reference Genes in Rat Pancreatic Islets Early after Isolation Affects Results of Gene Expression Studies

Lucie Kosinová,
Monika Cahová,
Eva Fábryová,
Irena Týcová,
Tomáš Koblas,
Ivan Leontovyč,
František Saudek,
Jan Kříž

Published: April 1, 2016
https://doi.org/10.1371/journal.pone.0152664

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Gene expression
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Insulin secretion
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Ribosomal RNA
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RNA isolation
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Dithizone staining
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Glucose metabolism
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Insulin
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Glucose
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