The Effect of Pericellular Oxygen Levels on Proteomic Profile and Lipogenesis in 3T3-L1 Differentiated Preadipocytes Cultured on Gas-Permeable Cultureware

Martin Weiszenstein; Nela Pavlikova; Moustafa Elkalaf; Petr Halada; Ondrej Seda; Jan Trnka; Jan Kovar; Jan Polak

doi:10.1371/journal.pone.0152382

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Peer-reviewed

Research Article

The Effect of Pericellular Oxygen Levels on Proteomic Profile and Lipogenesis in 3T3-L1 Differentiated Preadipocytes Cultured on Gas-Permeable Cultureware

Martin Weiszenstein,

Affiliation Center for Research on Diabetes, Metabolism and Nutrition, Third Faculty of Medicine, Charles University in Prague, Prague, Czech Republic
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Nela Pavlikova,

Affiliation Department of Biochemistry, Cell and Molecular Biology—Division of Cell and Molecular Biology, Third Faculty of Medicine, Charles University in Prague, Prague, Czech Republic
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Moustafa Elkalaf,

Affiliation Laboratory for Metabolism and Bioenergetics, Third Faculty of Medicine, Charles University in Prague, Prague, Czech Republic
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Petr Halada,

Affiliation Laboratory of Molecular Structure Characterization, Institute of Microbiology of the CAS, v.v.i., Prague, Czech Republic
⨯
Ondrej Seda,

Affiliation Institute of Biology and Medical Genetics, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic
⨯
Jan Trnka,

Affiliation Laboratory for Metabolism and Bioenergetics, Third Faculty of Medicine, Charles University in Prague, Prague, Czech Republic
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Jan Kovar,

Affiliation Department of Biochemistry, Cell and Molecular Biology—Division of Cell and Molecular Biology, Third Faculty of Medicine, Charles University in Prague, Prague, Czech Republic
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Jan Polak

* E-mail: jan.polak@lf3.cuni.cz

Affiliations Center for Research on Diabetes, Metabolism and Nutrition, Third Faculty of Medicine, Charles University in Prague, Prague, Czech Republic, Centre of Toxicology and Health Safety, The National Institute of Public Health, Prague, Czech Republic, 2nd Internal Medicine Department, University Hospital Kralovske Vinohrady, Prague, Czech Republic
⨯

The Effect of Pericellular Oxygen Levels on Proteomic Profile and Lipogenesis in 3T3-L1 Differentiated Preadipocytes Cultured on Gas-Permeable Cultureware

Martin Weiszenstein,
Nela Pavlikova,
Moustafa Elkalaf,
Petr Halada,
Ondrej Seda,
Jan Trnka,
Jan Kovar,
Jan Polak

Published: March 29, 2016
https://doi.org/10.1371/journal.pone.0152382

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Hypoxia
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Mitochondria
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Medical hypoxia
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Adipocytes
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Citric acid cycle
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Adipocyte differentiation
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Lipids
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Cell differentiation
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