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Open Access

Peer-reviewed

Research Article

Rapid and Robust PCR-Based All-Recombinant Cloning Methodology

Abhishek Anil Dubey ,

Contributed equally to this work with: Abhishek Anil Dubey, Manika Indrajit Singh

Affiliation Microbiology and Molecular Biology Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research (IISER), Bhopal, India
⨯
Manika Indrajit Singh ,

Contributed equally to this work with: Abhishek Anil Dubey, Manika Indrajit Singh

Affiliation Microbiology and Molecular Biology Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research (IISER), Bhopal, India
⨯
Vikas Jain

* E-mail: vikas@iiserb.ac.in

Affiliation Microbiology and Molecular Biology Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research (IISER), Bhopal, India
⨯

Rapid and Robust PCR-Based All-Recombinant Cloning Methodology

Abhishek Anil Dubey,
Manika Indrajit Singh,
Vikas Jain

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Published: March 23, 2016
https://doi.org/10.1371/journal.pone.0152106

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Polymerase chain reaction
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Vector cloning
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Cloning
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DNA cloning
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Gene cloning
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Protein expression
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Plasmid construction
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Recombination reactions
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