A Novel Approach for Ovine Primary Alveolar Epithelial Type II Cell Isolation and Culture from Fresh and Cryopreserved Tissue Obtained from Premature and Juvenile Animals

Mariola M. Marcinkiewicz; Sandy T. Baker; Jichuan Wu; Terrence L. Hubert; Marla R. Wolfson

doi:10.1371/journal.pone.0152027

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Research Article

A Novel Approach for Ovine Primary Alveolar Epithelial Type II Cell Isolation and Culture from Fresh and Cryopreserved Tissue Obtained from Premature and Juvenile Animals

Mariola M. Marcinkiewicz,

Affiliations Department of Thoracic Medicine and Surgery, Katz School of Medicine at Temple University, Philadelphia, PA, United States of America, Center for Inflammation, Translational and Clinical Lung Research, Katz School of Medicine at Temple University, Philadelphia, PA, United States of America, CENTRe: Collaborative for Environmental and Neonatal Therapeutics, Katz School of Medicine at Temple University, Philadelphia, PA, United States of America
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Sandy T. Baker,

Affiliations Department of Thoracic Medicine and Surgery, Katz School of Medicine at Temple University, Philadelphia, PA, United States of America, Center for Inflammation, Translational and Clinical Lung Research, Katz School of Medicine at Temple University, Philadelphia, PA, United States of America, CENTRe: Collaborative for Environmental and Neonatal Therapeutics, Katz School of Medicine at Temple University, Philadelphia, PA, United States of America
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Jichuan Wu,

Affiliations Department of Thoracic Medicine and Surgery, Katz School of Medicine at Temple University, Philadelphia, PA, United States of America, Center for Inflammation, Translational and Clinical Lung Research, Katz School of Medicine at Temple University, Philadelphia, PA, United States of America, CENTRe: Collaborative for Environmental and Neonatal Therapeutics, Katz School of Medicine at Temple University, Philadelphia, PA, United States of America
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Terrence L. Hubert,

Affiliations Department of Thoracic Medicine and Surgery, Katz School of Medicine at Temple University, Philadelphia, PA, United States of America, Center for Inflammation, Translational and Clinical Lung Research, Katz School of Medicine at Temple University, Philadelphia, PA, United States of America, CENTRe: Collaborative for Environmental and Neonatal Therapeutics, Katz School of Medicine at Temple University, Philadelphia, PA, United States of America
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Marla R. Wolfson

* E-mail: marla.wolfson@temple.edu

Affiliations Department of Thoracic Medicine and Surgery, Katz School of Medicine at Temple University, Philadelphia, PA, United States of America, Department of Physiology, Pediatrics and Medicine, Katz School of Medicine at Temple University, Philadelphia, PA, United States of America, Center for Inflammation, Translational and Clinical Lung Research, Katz School of Medicine at Temple University, Philadelphia, PA, United States of America, CENTRe: Collaborative for Environmental and Neonatal Therapeutics, Katz School of Medicine at Temple University, Philadelphia, PA, United States of America
⨯

A Novel Approach for Ovine Primary Alveolar Epithelial Type II Cell Isolation and Culture from Fresh and Cryopreserved Tissue Obtained from Premature and Juvenile Animals

Mariola M. Marcinkiewicz,
Sandy T. Baker,
Jichuan Wu,
Terrence L. Hubert,
Marla R. Wolfson

Published: March 21, 2016
https://doi.org/10.1371/journal.pone.0152027

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