Sensitive Visual Detection of AHPND Bacteria Using Loop-Mediated Isothermal Amplification Combined with DNA-Functionalized Gold Nanoparticles as Probes

Narong Arunrut; Jantana Kampeera; Sarawut Sirithammajak; Piyachat Sanguanrut; Porranee Proespraiwong; Rungkarn Suebsing; Wansika Kiatpathomchai

doi:10.1371/journal.pone.0151769

Loading metrics

Open Access

Peer-reviewed

Research Article

Sensitive Visual Detection of AHPND Bacteria Using Loop-Mediated Isothermal Amplification Combined with DNA-Functionalized Gold Nanoparticles as Probes

Narong Arunrut,

Affiliation Bioengineering and Sensing Technology Laboratory, BIOTEC, National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Phahonyothin Rd., Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand
⨯
Jantana Kampeera,

Affiliation Bioengineering and Sensing Technology Laboratory, BIOTEC, National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Phahonyothin Rd., Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand
⨯
Sarawut Sirithammajak,

Affiliation Bioengineering and Sensing Technology Laboratory, BIOTEC, National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Phahonyothin Rd., Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand
⨯
Piyachat Sanguanrut,

Affiliation Center of Excellence for Shrimp Molecular Biology and Biotechnology (CENTEX Shrimp), Faculty of Science, Mahidol University, Rama VI road, Bangkok 10400, Thailand
⨯
Porranee Proespraiwong,

Affiliation Aquatic Animal Health Research Center, Charoen Pokphand Foods Pulic CO., LTD. Samutsakorn 74000, Thailand
⨯
Rungkarn Suebsing,

Affiliation Bioengineering and Sensing Technology Laboratory, BIOTEC, National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Phahonyothin Rd., Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand
⨯
Wansika Kiatpathomchai

* E-mail: wansika@biotec.or.th

Affiliation Bioengineering and Sensing Technology Laboratory, BIOTEC, National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Phahonyothin Rd., Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand
⨯

Sensitive Visual Detection of AHPND Bacteria Using Loop-Mediated Isothermal Amplification Combined with DNA-Functionalized Gold Nanoparticles as Probes

Narong Arunrut,
Jantana Kampeera,
Sarawut Sirithammajak,
Piyachat Sanguanrut,
Porranee Proespraiwong,
Rungkarn Suebsing,
Wansika Kiatpathomchai

Published: March 22, 2016
https://doi.org/10.1371/journal.pone.0151769

Reader Comments (0)

Post a new comment on this article

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

Shrimp
Is the Subject Area "Shrimp" applicable to this article?

Thanks for your feedback.
Polymerase chain reaction
Is the Subject Area "Polymerase chain reaction" applicable to this article?

Thanks for your feedback.
DNA extraction
Is the Subject Area "DNA extraction" applicable to this article?

Thanks for your feedback.
DNA isolation
Is the Subject Area "DNA isolation" applicable to this article?

Thanks for your feedback.
Nested polymerase chain reaction
Is the Subject Area "Nested polymerase chain reaction" applicable to this article?

Thanks for your feedback.
Probe hybridization
Is the Subject Area "Probe hybridization" applicable to this article?

Thanks for your feedback.
Toxins
Is the Subject Area "Toxins" applicable to this article?

Thanks for your feedback.
Ponds
Is the Subject Area "Ponds" applicable to this article?

Thanks for your feedback.