When Phase Contrast Fails: ChainTracer and NucTracer, Two ImageJ Methods for Semi-Automated Single Cell Analysis Using Membrane or DNA Staining

Open Access

Peer-reviewed

Research Article

Simon Syvertsson,

Affiliation Centre for Bacterial Cell Biology, Newcastle University, Newcastle upon Tyne, Richardson Road, Newcastle, NE2 4AX, United Kingdom
⨯
Norbert O. E. Vischer,

Affiliation Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands
⨯
Yongqiang Gao,

Affiliation Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands
⨯
Leendert W. Hamoen

* E-mail: l.w.hamoen@uva.nl

Affiliations Centre for Bacterial Cell Biology, Newcastle University, Newcastle upon Tyne, Richardson Road, Newcastle, NE2 4AX, United Kingdom, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands
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Membrane staining
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Cell staining
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Bacillus subtilis
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Fluorescence imaging
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Pathogen motility
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Phase contrast microscopy
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Bacteria
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Lactococcus lactis
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