In Vitro Optimization of Enzymes Involved in Precorrin-2 Synthesis Using Response Surface Methodology

Open Access

Peer-reviewed

Research Article

Huan Fang,

Affiliations Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China, Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin, China, University of Chinese Academy of Sciences, Beijing, China
⨯
Huina Dong,

Affiliations Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China, Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin, China
⨯
Tao Cai,

Affiliation Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China
⨯
Ping Zheng,

Affiliations Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China, Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin, China
⨯
Haixing Li,

Affiliation Sino-German Joint Research Institute, Nanchang University, Nanchang, Jiangxi, China
⨯
Dawei Zhang ,

* E-mail: zhang_dw@tib.cas.cn

Affiliations Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China, Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin, China
⨯
Jibin Sun

Affiliations Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China, Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin, China
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Enzymes
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Chemical synthesis
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Cobalamins
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Dehydrogenases
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Biochemical cofactors
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Reaction systems
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Enzyme inhibitors
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Plasmid construction
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