EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple Genomic Integrations in Saccharomyces cerevisiae

Jérôme Maury; Susanne M. Germann; Simo Abdessamad Baallal Jacobsen; Niels B. Jensen; Kanchana R. Kildegaard; Markus J. Herrgård; Konstantin Schneider; Anna Koza; Jochen Forster; Jens Nielsen; Irina Borodina

doi:10.1371/journal.pone.0150394

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Open Access

Peer-reviewed

Research Article

EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple Genomic Integrations in Saccharomyces cerevisiae

Jérôme Maury ,

Contributed equally to this work with: Jérôme Maury, Susanne M. Germann

* E-mail: jmau@biosustain.dtu.dk

Affiliation The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark
⨯
Susanne M. Germann ,

Contributed equally to this work with: Jérôme Maury, Susanne M. Germann

Current Address: Evolva SA, Copenhagen, Denmark

Affiliation The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark
⨯
Simo Abdessamad Baallal Jacobsen,

Affiliation The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark
⨯
Niels B. Jensen,

Current Address: Evolva SA, Copenhagen, Denmark

Affiliation The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark
⨯
Kanchana R. Kildegaard,

Affiliation The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark
⨯
Markus J. Herrgård,

Affiliation The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark
⨯
Konstantin Schneider,

Affiliation The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark
⨯
Anna Koza,

Affiliation The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark
⨯
Jochen Forster,

Affiliation The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark
⨯
Jens Nielsen,

Affiliations The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark, Department of Biology and Biological Engineering, Chalmers University of Technology, Göteborg, Sweden
⨯
Irina Borodina

Affiliation The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark
⨯

EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple Genomic Integrations in Saccharomyces cerevisiae

Jérôme Maury,
Susanne M. Germann,
Simo Abdessamad Baallal Jacobsen,
Niels B. Jensen,
Kanchana R. Kildegaard,
Markus J. Herrgård,
Konstantin Schneider,
Anna Koza,
Jochen Forster,
Jens Nielsen

Published: March 2, 2016
https://doi.org/10.1371/journal.pone.0150394

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Saccharomyces cerevisiae
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Genomics
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Genetic loci
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Cloning
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Vector cloning
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Homologous recombination
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Polymerase chain reaction
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Vector construction
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