Development of a Genus-Specific Antigen Capture ELISA for Orthopoxviruses – Target Selection and Optimized Screening

Daniel Stern; Diana Pauly; Martin Zydek; Lilija Miller; Janett Piesker; Michael Laue; Fred Lisdat; Martin B. Dorner; Brigitte G. Dorner; Andreas Nitsche

doi:10.1371/journal.pone.0150110

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Open Access

Peer-reviewed

Research Article

Development of a Genus-Specific Antigen Capture ELISA for Orthopoxviruses – Target Selection and Optimized Screening

Daniel Stern ,

* E-mail: SternD@rki.de

Affiliation Highly Pathogenic Viruses (ZBS 1), Centre for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin, Germany
⨯
Diana Pauly,

Current address: Department of Ophthalmology, University Hospital Regensburg, Regensburg, Germany

Affiliation Biological Toxins (ZBS 3), Centre for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin, Germany
⨯
Martin Zydek,

Current address: Department of Cell and Tissue Biology, University of California San Francisco, San Francisco, CA, United States of America

Affiliation Biosystems Technology, Institute of Applied Life Sciences, Technical University of Applied Sciences, Wildau, Germany
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Lilija Miller,

Current address: Junior Research Group 2, Novel Vaccination Strategies and Early Immune Responses, Paul-Ehrlich-Institut, Langen, Germany

Affiliation Highly Pathogenic Viruses (ZBS 1), Centre for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin, Germany
⨯
Janett Piesker,

Affiliation Advanced Light and Electron Microscopy (ZBS 4), Centre for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin, Germany
⨯
Michael Laue,

Affiliation Advanced Light and Electron Microscopy (ZBS 4), Centre for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin, Germany
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Fred Lisdat,

Affiliation Biosystems Technology, Institute of Applied Life Sciences, Technical University of Applied Sciences, Wildau, Germany
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Martin B. Dorner,

Affiliation Biological Toxins (ZBS 3), Centre for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin, Germany
⨯
Brigitte G. Dorner,

Affiliation Biological Toxins (ZBS 3), Centre for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin, Germany
⨯
Andreas Nitsche

Affiliation Highly Pathogenic Viruses (ZBS 1), Centre for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin, Germany
⨯

Development of a Genus-Specific Antigen Capture ELISA for Orthopoxviruses – Target Selection and Optimized Screening

Daniel Stern,
Diana Pauly,
Martin Zydek,
Lilija Miller,
Janett Piesker,
Michael Laue,
Fred Lisdat,
Martin B. Dorner,
Brigitte G. Dorner,
Andreas Nitsche

Published: March 1, 2016
https://doi.org/10.1371/journal.pone.0150110

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