PCR-Based Seamless Genome Editing with High Efficiency and Fidelity in Escherichia coli

Yilan Liu; Maohua Yang; Jinjin Chen; Daojiang Yan; Wanwan Cheng; Yanyan Wang; Anders Thygesen; Ruonan Chen; Jianmin Xing; Qinhong Wang; Yanhe Ma

doi:10.1371/journal.pone.0149762

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Open Access

Peer-reviewed

Research Article

PCR-Based Seamless Genome Editing with High Efficiency and Fidelity in Escherichia coli

Yilan Liu,

Affiliation Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 XiQiDao, Tianjin Airport Economic Area, Tianjin, 300308, China
⨯
Maohua Yang,

Affiliation National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, PR China
⨯
Jinjin Chen,

Affiliation National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, PR China
⨯
Daojiang Yan,

Affiliation National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, PR China
⨯
Wanwan Cheng,

Affiliation National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, PR China
⨯
Yanyan Wang,

Affiliation National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, PR China
⨯
Anders Thygesen,

Affiliation Center of Bioprocess Engineering, Department of Chemical and Biochemical Engineering, Technical University of Denmark, DK-2800, Lyngby, Denmark
⨯
Ruonan Chen,

Affiliation National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, PR China
⨯
Jianmin Xing ,

* E-mail: jmxing@ipe.ac.cn (JX); wang_qh@tib.cas.cn (QW)

Affiliation National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, PR China
⨯
Qinhong Wang ,

* E-mail: jmxing@ipe.ac.cn (JX); wang_qh@tib.cas.cn (QW)

Affiliation Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 XiQiDao, Tianjin Airport Economic Area, Tianjin, 300308, China
⨯
Yanhe Ma

Affiliation Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 XiQiDao, Tianjin Airport Economic Area, Tianjin, 300308, China
⨯

PCR-Based Seamless Genome Editing with High Efficiency and Fidelity in Escherichia coli

Yilan Liu,
Maohua Yang,
Jinjin Chen,
Daojiang Yan,
Wanwan Cheng,
Yanyan Wang,
Anders Thygesen,
Ruonan Chen,
Jianmin Xing,
Qinhong Wang

Published: March 28, 2016
https://doi.org/10.1371/journal.pone.0149762

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