Identification of Essential Genetic Baculoviral Elements for Recombinant Protein Expression by Transactivation in Sf21 Insect Cells

Maren Bleckmann; Margitta Schürig; Fang-Fang Chen; Zen-Zen Yen; Nils Lindemann; Steffen Meyer; Johannes Spehr; Joop van den Heuvel

doi:10.1371/journal.pone.0149424

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Open Access

Peer-reviewed

Research Article

Identification of Essential Genetic Baculoviral Elements for Recombinant Protein Expression by Transactivation in Sf21 Insect Cells

Maren Bleckmann ,

Contributed equally to this work with: Maren Bleckmann, Margitta Schürig

Affiliation Department of Structure and Function of Proteins, Research Group Recombinant Protein Expression, Helmholtz Centre for Infection Research, Braunschweig, Germany
⨯
Margitta Schürig ,

Contributed equally to this work with: Maren Bleckmann, Margitta Schürig

Affiliation Department of Structure and Function of Proteins, Research Group Recombinant Protein Expression, Helmholtz Centre for Infection Research, Braunschweig, Germany
⨯
Fang-Fang Chen,

Affiliation Department of Structure and Function of Proteins, Research Group Recombinant Protein Expression, Helmholtz Centre for Infection Research, Braunschweig, Germany
⨯
Zen-Zen Yen,

Affiliation Department of Structure and Function of Proteins, Research Group Recombinant Protein Expression, Helmholtz Centre for Infection Research, Braunschweig, Germany
⨯
Nils Lindemann,

Affiliation Department of Structure and Function of Proteins, Research Group Recombinant Protein Expression, Helmholtz Centre for Infection Research, Braunschweig, Germany
⨯
Steffen Meyer,

Affiliation Department of Structure and Function of Proteins, Research Group Recombinant Protein Expression, Helmholtz Centre for Infection Research, Braunschweig, Germany
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Johannes Spehr,

Affiliation Department of Structure and Function of Proteins, Research Group Recombinant Protein Expression, Helmholtz Centre for Infection Research, Braunschweig, Germany
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Joop van den Heuvel

* E-mail: Joop.vandenHeuvel@helmholtz-hzi.de

Affiliation Department of Structure and Function of Proteins, Research Group Recombinant Protein Expression, Helmholtz Centre for Infection Research, Braunschweig, Germany
⨯

Identification of Essential Genetic Baculoviral Elements for Recombinant Protein Expression by Transactivation in Sf21 Insect Cells

Maren Bleckmann,
Margitta Schürig,
Fang-Fang Chen,
Zen-Zen Yen,
Nils Lindemann,
Steffen Meyer,
Johannes Spehr,
Joop van den Heuvel

Published: March 2, 2016
https://doi.org/10.1371/journal.pone.0149424

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Transactivation
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Transfection
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Insects
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Recombinant proteins
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