Comparison of Sample Preparation Methods Used for the Next-Generation Sequencing of Mycobacterium tuberculosis

Andrea D. Tyler; Sara Christianson; Natalie C. Knox; Philip Mabon; Joyce Wolfe; Gary Van Domselaar; Morag R. Graham; Meenu K. Sharma

doi:10.1371/journal.pone.0148676

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Open Access

Peer-reviewed

Research Article

Comparison of Sample Preparation Methods Used for the Next-Generation Sequencing of Mycobacterium tuberculosis

Andrea D. Tyler ,

* E-mail: andrea.d.tyler@phac-aspc.gc.ca (ADT); meenu.sharma@phac-aspc.gc.ca (MKS)

Affiliation National Microbiology Laboratory, National Reference Centre for Mycobacteriology, Public Health Agency of Canada, Winnipeg, Manitoba, Canada
⨯
Sara Christianson,

Affiliation National Microbiology Laboratory, National Reference Centre for Mycobacteriology, Public Health Agency of Canada, Winnipeg, Manitoba, Canada
⨯
Natalie C. Knox,

Affiliation Science Technology Cores & Services Division, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada
⨯
Philip Mabon,

Affiliation Science Technology Cores & Services Division, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada
⨯
Joyce Wolfe,

Affiliation National Microbiology Laboratory, National Reference Centre for Mycobacteriology, Public Health Agency of Canada, Winnipeg, Manitoba, Canada
⨯
Gary Van Domselaar,

Affiliations Science Technology Cores & Services Division, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada, Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada, Department of Computer Science, University of Manitoba, Winnipeg, Manitoba, Canada
⨯
Morag R. Graham,

Affiliations Science Technology Cores & Services Division, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada, Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada
⨯
Meenu K. Sharma

* E-mail: andrea.d.tyler@phac-aspc.gc.ca (ADT); meenu.sharma@phac-aspc.gc.ca (MKS)

Affiliations National Microbiology Laboratory, National Reference Centre for Mycobacteriology, Public Health Agency of Canada, Winnipeg, Manitoba, Canada, Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada
⨯

Comparison of Sample Preparation Methods Used for the Next-Generation Sequencing of Mycobacterium tuberculosis

Andrea D. Tyler,
Sara Christianson,
Natalie C. Knox,
Philip Mabon,
Joyce Wolfe,
Gary Van Domselaar,
Morag R. Graham,
Meenu K. Sharma

Published: February 5, 2016
https://doi.org/10.1371/journal.pone.0148676

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Mycobacterium tuberculosis
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Genomics
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Genome sequencing
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Sequence motif analysis
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Bacterial genomics
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DNA sequencing
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DNA isolation
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