Mathematical Model of the Firefly Luciferase Complementation Assay Reveals a Non-Linear Relationship between the Detected Luminescence and the Affinity of the Protein Pair Being Analyzed

Open Access

Peer-reviewed

Research Article

Renee Dale,

Affiliation Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana, United States of America
⨯
Yuki Ohmuro-Matsuyama,

Affiliation Chemical Resources Laboratory, Tokyo Institute of Technology, Nagatsuta-cho, Yokohama, Kanagawa, Japan
⨯
Hiroshi Ueda,

Affiliation Chemical Resources Laboratory, Tokyo Institute of Technology, Nagatsuta-cho, Yokohama, Kanagawa, Japan
⨯
Naohiro Kato

* E-mail: kato@lsu.edu

Affiliation Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana, United States of America
⨯

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Luciferase
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Luminescence
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Curve fitting
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Mathematical models
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Light
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Protein interactions
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Oxidation
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Luciferase assay
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