Critical Parameters for Efficient Sonication and Improved Chromatin Immunoprecipitation of High Molecular Weight Proteins

Open Access

Peer-reviewed

Research Article

Nikolay A. Pchelintsev ,

* E-mail: nikolay.pchelintsev@abcam.com

Current address: Abcam plc, 204 Cambridge Science Park, Cambridge, United Kingdom

Affiliation CR-UK Beatson Labs, Institute of Cancer Sciences, University of Glasgow, Glasgow, United Kingdom
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Peter D. Adams,

Affiliation CR-UK Beatson Labs, Institute of Cancer Sciences, University of Glasgow, Glasgow, United Kingdom
⨯
David M. Nelson

Affiliation CR-UK Beatson Labs, Institute of Cancer Sciences, University of Glasgow, Glasgow, United Kingdom
⨯

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Sonication
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Chromatin
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DNA purification
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Cross-linking
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Gel electrophoresis
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Polymerase chain reaction
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Immunoprecipitation
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Lysis (medicine)
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