Development of Real-Time PCR Methods for the Detection of Bacterial Meningitis Pathogens without DNA Extraction

• Jeni Vuong,

  Affiliation Meningitis and Vaccine Preventable Diseases Branch, Centers for Disease Control and Prevention, Atlanta, United States of America

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• Jean-Marc Collard,

  Affiliations Scientific Institute of Public Health, Brussels, Belgium, Centre de Recherche Médicale et Sanitaire, Niamey, Niger

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• Melissa J. Whaley,

  Affiliation Meningitis and Vaccine Preventable Diseases Branch, Centers for Disease Control and Prevention, Atlanta, United States of America

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• Issaka Bassira,

  Affiliation Centre de Recherche Médicale et Sanitaire, Niamey, Niger

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• Issaka Seidou,

  Affiliation Centre de Recherche Médicale et Sanitaire, Niamey, Niger

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• Seydou Diarra,

  Affiliation Institut National de Recherche en Santé Publique, Bamako, Mali

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• Rasmata T. Ouédraogo,

  Affiliation Centre Hospitalier Universitaire Pédiatrique Charles De Gaulle, Ouagadougou, Burkina Faso

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• Dinanibè Kambiré,

  Affiliation Centre Hospitalier Universitaire Pédiatrique Charles De Gaulle, Ouagadougou, Burkina Faso

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• Thomas H. Taylor Jr,

  Affiliation Meningitis and Vaccine Preventable Diseases Branch, Centers for Disease Control and Prevention, Atlanta, United States of America

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• Claudio Sacchi,

  Affiliation Instituto Adolfo Lutz, São Paulo, Brazil

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• Leonard W. Mayer,

  Affiliation Meningitis and Vaccine Preventable Diseases Branch, Centers for Disease Control and Prevention, Atlanta, United States of America

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• Xin Wang

  * E-mail: gqe8@cdc.gov

  Affiliation Meningitis and Vaccine Preventable Diseases Branch, Centers for Disease Control and Prevention, Atlanta, United States of America

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