Widefield Two-Photon Excitation without Scanning: Live Cell Microscopy with High Time Resolution and Low Photo-Bleaching

Rumelo Amor; Alison McDonald; Johanna Trägårdh; Gillian Robb; Louise Wilson; Nor Zaihana Abdul Rahman; John Dempster; William Bradshaw Amos; Trevor J. Bushell; Gail McConnell

doi:10.1371/journal.pone.0147115

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Open Access

Peer-reviewed

Research Article

Widefield Two-Photon Excitation without Scanning: Live Cell Microscopy with High Time Resolution and Low Photo-Bleaching

Rumelo Amor ,

* E-mail: rumelo.amor@gmail.com

Affiliation Centre for Biophotonics, Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom
⨯
Alison McDonald,

Affiliation Centre for Biophotonics, Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom
⨯
Johanna Trägårdh,

Affiliation Centre for Biophotonics, Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom
⨯
Gillian Robb,

Affiliation Centre for Biophotonics, Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom
⨯
Louise Wilson,

Affiliation Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom
⨯
Nor Zaihana Abdul Rahman,

Affiliation Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom
⨯
John Dempster,

Affiliation Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom
⨯
William Bradshaw Amos,

Affiliations Centre for Biophotonics, Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom, MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, United Kingdom
⨯
Trevor J. Bushell,

Affiliation Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom
⨯
Gail McConnell

Affiliation Centre for Biophotonics, Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom
⨯

Widefield Two-Photon Excitation without Scanning: Live Cell Microscopy with High Time Resolution and Low Photo-Bleaching

Rumelo Amor,
Alison McDonald,
Johanna Trägårdh,
Gillian Robb,
Louise Wilson,
Nor Zaihana Abdul Rahman,
John Dempster,
William Bradshaw Amos,
Trevor J. Bushell,
Gail McConnell

Published: January 29, 2016
https://doi.org/10.1371/journal.pone.0147115

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