Epithelial-to-Mesenchymal Transition of RPE Cells In Vitro Confers Increased β1,6-N-Glycosylation and Increased Susceptibility to Galectin-3 Binding

Claudia S. Priglinger; Jara Obermann; Christoph M. Szober; Juliane Merl-Pham; Uli Ohmayer; Jennifer Behler; Fabian Gruhn; Thomas C. Kreutzer; Christian Wertheimer; Arie Geerlof; Siegfried G. Priglinger; Stefanie M. Hauck

doi:10.1371/journal.pone.0146887

Loading metrics

Open Access

Peer-reviewed

Research Article

Epithelial-to-Mesenchymal Transition of RPE Cells In Vitro Confers Increased β1,6-N-Glycosylation and Increased Susceptibility to Galectin-3 Binding

Claudia S. Priglinger ,

* E-mail: claudia.alge-priglinger@med.uni-muenchen.de

Affiliation Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany
⨯
Jara Obermann,

Affiliation Research Unit Protein Science, Helmholtz Zentrum Munich, German Research Center for Environmental Health (GmbH), Munich, Germany
⨯
Christoph M. Szober,

Affiliation Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany
⨯
Juliane Merl-Pham,

Affiliation Research Unit Protein Science, Helmholtz Zentrum Munich, German Research Center for Environmental Health (GmbH), Munich, Germany
⨯
Uli Ohmayer,

Affiliation Research Unit Protein Science, Helmholtz Zentrum Munich, German Research Center for Environmental Health (GmbH), Munich, Germany
⨯
Jennifer Behler,

Affiliation Research Unit Protein Science, Helmholtz Zentrum Munich, German Research Center for Environmental Health (GmbH), Munich, Germany
⨯
Fabian Gruhn,

Affiliation Research Unit Protein Science, Helmholtz Zentrum Munich, German Research Center for Environmental Health (GmbH), Munich, Germany
⨯
Thomas C. Kreutzer,

Affiliation Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany
⨯
Christian Wertheimer,

Affiliation Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany
⨯
Arie Geerlof,

Affiliation Protein Expression and Purification Facility, Institute of Structural Biology, Helmholtz Zentrum Munich, German Research Center for Environmental Health (GmbH), Neuherberg, Germany
⨯
Siegfried G. Priglinger,

Affiliation Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany
⨯
Stefanie M. Hauck

Affiliation Research Unit Protein Science, Helmholtz Zentrum Munich, German Research Center for Environmental Health (GmbH), Munich, Germany
⨯

Epithelial-to-Mesenchymal Transition of RPE Cells In Vitro Confers Increased β1,6-N-Glycosylation and Increased Susceptibility to Galectin-3 Binding

Claudia S. Priglinger,
Jara Obermann,
Christoph M. Szober,
Juliane Merl-Pham,
Uli Ohmayer,
Jennifer Behler,
Fabian Gruhn,
Thomas C. Kreutzer,
Christian Wertheimer,
Arie Geerlof

Published: January 13, 2016
https://doi.org/10.1371/journal.pone.0146887

Reader Comments (0)

Post a new comment on this article

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

Cell binding
Is the Subject Area "Cell binding" applicable to this article?

Thanks for your feedback.
Lectins
Is the Subject Area "Lectins" applicable to this article?

Thanks for your feedback.
Eyes
Is the Subject Area "Eyes" applicable to this article?

Thanks for your feedback.
Cell binding assay
Is the Subject Area "Cell binding assay" applicable to this article?

Thanks for your feedback.
Transfection
Is the Subject Area "Transfection" applicable to this article?

Thanks for your feedback.
Small interfering RNA
Is the Subject Area "Small interfering RNA" applicable to this article?

Thanks for your feedback.
Cell staining
Is the Subject Area "Cell staining" applicable to this article?

Thanks for your feedback.
Sialic acids
Is the Subject Area "Sialic acids" applicable to this article?

Thanks for your feedback.