A New Generation of FRET Sensors for Robust Measurement of Gαi1, Gαi2 and Gαi3 Activation Kinetics in Single Cells

Jakobus van Unen; Anette D. Stumpf; Benedikt Schmid; Nathalie R. Reinhard; Peter L. Hordijk; Carsten Hoffmann; Theodorus W. J. Gadella Jr.; Joachim Goedhart

doi:10.1371/journal.pone.0146789

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Open Access

Peer-reviewed

Research Article

A New Generation of FRET Sensors for Robust Measurement of Gα_i1, Gα_i2 and Gα_i3 Activation Kinetics in Single Cells

Jakobus van Unen,

Affiliation Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, P.O. Box 94215, NL-1090 GE, Amsterdam, The Netherlands
⨯
Anette D. Stumpf,

Affiliation Bio-Imaging-Center/Rudolf-Virchow-Zentrum and Department of Pharmacology and Toxicology, University of Wuerzburg, Versbacher Strasse 9, 97078, Wuerzburg, Germany
⨯
Benedikt Schmid,

Affiliation Bio-Imaging-Center/Rudolf-Virchow-Zentrum and Department of Pharmacology and Toxicology, University of Wuerzburg, Versbacher Strasse 9, 97078, Wuerzburg, Germany
⨯
Nathalie R. Reinhard,

Affiliations Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, P.O. Box 94215, NL-1090 GE, Amsterdam, The Netherlands, Department of Molecular Cell Biology, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, NL-1066 CX, Amsterdam, the Netherlands
⨯
Peter L. Hordijk,

Affiliations Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, P.O. Box 94215, NL-1090 GE, Amsterdam, The Netherlands, Department of Molecular Cell Biology, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, NL-1066 CX, Amsterdam, the Netherlands
⨯
Carsten Hoffmann,

Affiliation Bio-Imaging-Center/Rudolf-Virchow-Zentrum and Department of Pharmacology and Toxicology, University of Wuerzburg, Versbacher Strasse 9, 97078, Wuerzburg, Germany
⨯
Theodorus W. J. Gadella Jr.,

Affiliation Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, P.O. Box 94215, NL-1090 GE, Amsterdam, The Netherlands
⨯
Joachim Goedhart

* E-mail: j.goedhart@uva.nl

Affiliation Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, P.O. Box 94215, NL-1090 GE, Amsterdam, The Netherlands
⨯

A New Generation of FRET Sensors for Robust Measurement of Gα_i1, Gα_i2 and Gα_i3 Activation Kinetics in Single Cells

Jakobus van Unen,
Anette D. Stumpf,
Benedikt Schmid,
Nathalie R. Reinhard,
Peter L. Hordijk,
Carsten Hoffmann,
Theodorus W. J. Gadella Jr.,
Joachim Goedhart

Published: January 22, 2016
https://doi.org/10.1371/journal.pone.0146789

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