Engineering of a Bacillus amyloliquefaciens Strain with High Neutral Protease Producing Capacity and Optimization of Its Fermentation Conditions

Open Access

Peer-reviewed

Research Article

Hui Wang,

Affiliation Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P. R. China
⨯
Lian Yang,

Affiliation Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P. R. China
⨯
Yanhai Ping,

Affiliation Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P. R. China
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Yingguo Bai,

Affiliation Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P. R. China
⨯
Huiying Luo,

Affiliation Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P. R. China
⨯
Huoqing Huang ,

* E-mail: huanghuoqing@caas.cn (HH); binyao@caas.cn (BY)

Affiliation Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P. R. China
⨯
Bin Yao

* E-mail: huanghuoqing@caas.cn (HH); binyao@caas.cn (BY)

Affiliation Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P. R. China
⨯

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Bacillus subtilis
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Proteases
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Plasmid construction
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Fermentation
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Bacillus
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Bacillus cereus
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DNA recombination
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Transformation associated recombination
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