Human Gut-Commensalic Lactobacillus ruminis ATCC 25644 Displays Sortase-Assembled Surface Piliation: Phenotypic Characterization of Its Fimbrial Operon through In Silico Predictive Analysis and Recombinant Expression in Lactococcus lactis

Xia Yu; Annukka Jaatinen; Johanna Rintahaka; Ulla Hynönen; Outi Lyytinen; Ravi Kant; Silja Åvall-Jääskeläinen; Ingemar von Ossowski; Airi Palva

doi:10.1371/journal.pone.0145718

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Research Article

Human Gut-Commensalic Lactobacillus ruminis ATCC 25644 Displays Sortase-Assembled Surface Piliation: Phenotypic Characterization of Its Fimbrial Operon through In Silico Predictive Analysis and Recombinant Expression in Lactococcus lactis

Xia Yu,

Affiliation Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland
⨯
Annukka Jaatinen ,

Contributed equally to this work with: Annukka Jaatinen, Johanna Rintahaka, Ulla Hynönen

Affiliation Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland
⨯
Johanna Rintahaka ,

Contributed equally to this work with: Annukka Jaatinen, Johanna Rintahaka, Ulla Hynönen

Affiliation Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland
⨯
Ulla Hynönen ,

Contributed equally to this work with: Annukka Jaatinen, Johanna Rintahaka, Ulla Hynönen

Affiliation Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland
⨯
Outi Lyytinen,

Affiliation Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland
⨯
Ravi Kant,

Affiliation Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland
⨯
Silja Åvall-Jääskeläinen,

Affiliation Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland
⨯
Ingemar von Ossowski ,

* E-mail: ingemar.von.ossowski@helsinki.fi (IvO); airi.palva@helsinki.fi (AP)

Affiliation Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland
⨯
Airi Palva

* E-mail: ingemar.von.ossowski@helsinki.fi (IvO); airi.palva@helsinki.fi (AP)

Affiliation Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland
⨯

Human Gut-Commensalic Lactobacillus ruminis ATCC 25644 Displays Sortase-Assembled Surface Piliation: Phenotypic Characterization of Its Fimbrial Operon through In Silico Predictive Analysis and Recombinant Expression in Lactococcus lactis

Xia Yu,
Annukka Jaatinen,
Johanna Rintahaka,
Ulla Hynönen,
Outi Lyytinen,
Ravi Kant,
Silja Åvall-Jääskeläinen,
Ingemar von Ossowski,
Airi Palva

Published: December 28, 2015
https://doi.org/10.1371/journal.pone.0145718

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Pili and fimbriae
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Cloning
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Lactococcus
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Operons
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Lactococcus lactis
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Immune serum
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