Single-Tubed Wild-Type Blocking Quantitative PCR Detection Assay for the Sensitive Detection of Codon 12 and 13 KRAS Mutations

Jun-Fu Huang; Dong-Zhu Zeng; Guang-Jie Duan; Yan Shi; Guo-Hong Deng; Han Xia; Han-Qing Xu; Na Zhao; Wei-Ling Fu; Qing Huang

doi:10.1371/journal.pone.0145698

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Open Access

Peer-reviewed

Research Article

Single-Tubed Wild-Type Blocking Quantitative PCR Detection Assay for the Sensitive Detection of Codon 12 and 13 KRAS Mutations

Jun-Fu Huang ,

Contributed equally to this work with: Jun-Fu Huang, Dong-Zhu Zeng

Affiliation Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing, 400038, P. R. China
⨯
Dong-Zhu Zeng ,

Contributed equally to this work with: Jun-Fu Huang, Dong-Zhu Zeng

Affiliation Department of General Surgery, Southwest Hospital, Third Military Medical University, Chongqing, 400038, P. R. China
⨯
Guang-Jie Duan,

Affiliation Institute of Pathology and Southwest Cancer Center, Southwest Hospital, Third Military Medical University, Chongqing, 400038, P. R. China
⨯
Yan Shi,

Affiliation Department of General Surgery, Southwest Hospital, Third Military Medical University, Chongqing, 400038, P. R. China
⨯
Guo-Hong Deng,

Affiliation Department of Infectious Diseases, Southwest Hospital, Third Military Medical University, Chongqing, 400038, P. R. China
⨯
Han Xia,

Affiliation Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing, 400038, P. R. China
⨯
Han-Qing Xu,

Affiliation Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing, 400038, P. R. China
⨯
Na Zhao,

Affiliation Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing, 400038, P. R. China
⨯
Wei-Ling Fu ,

* E-mail: Q.Huang@gmail.com (QH); weilingfu@yahoo.com (W-LF)

Affiliation Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing, 400038, P. R. China
⨯
Qing Huang

* E-mail: Q.Huang@gmail.com (QH); weilingfu@yahoo.com (W-LF)

Affiliation Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing, 400038, P. R. China
⨯

Single-Tubed Wild-Type Blocking Quantitative PCR Detection Assay for the Sensitive Detection of Codon 12 and 13 KRAS Mutations

Jun-Fu Huang,
Dong-Zhu Zeng,
Guang-Jie Duan,
Yan Shi,
Guo-Hong Deng,
Han Xia,
Han-Qing Xu,
Na Zhao,
Wei-Ling Fu,
Qing Huang

Published: December 23, 2015
https://doi.org/10.1371/journal.pone.0145698

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Polymerase chain reaction
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Mutation detection
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DNA extraction
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DNA isolation
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DNA polymerase
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Oligonucleotides
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