Rational Design of High-Number dsDNA Fragments Based on Thermodynamics for the Construction of Full-Length Genes in a Single Reaction

Open Access

Peer-reviewed

Research Article

Bhagyashree S. Birla,

Affiliation Department of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, United States of America
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Hui-Hsien Chou

* E-mail: hhchou@iastate.edu

Affiliations Department of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, United States of America, Department of Computer Science, Iowa State University, Ames, Iowa, United States of America
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Molecular cloning
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DNA annealing
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Oligonucleotides
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Polymerase chain reaction
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DNA
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Reaction time
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Thermodynamics
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