Exploiting the CRISPR/Cas9 PAM Constraint for Single-Nucleotide Resolution Interventions

Yi Li; Saurabh Mendiratta; Kristina Ehrhardt; Neha Kashyap; Michael A. White; Leonidas Bleris

doi:10.1371/journal.pone.0144970

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Peer-reviewed

Research Article

Exploiting the CRISPR/Cas9 PAM Constraint for Single-Nucleotide Resolution Interventions

Yi Li,

Affiliations Bioengineering Department, The University of Texas at Dallas, 800 West Campbell Road, Richardson, Texas 75080, United States of America, Center for Systems Biology, The University of Texas at Dallas, 800 West Campbell Road, Richardson, Texas 75080, United States of America
⨯
Saurabh Mendiratta,

Affiliation Department of Cell Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, Texas 75390, United States of America
⨯
Kristina Ehrhardt,

Affiliations Bioengineering Department, The University of Texas at Dallas, 800 West Campbell Road, Richardson, Texas 75080, United States of America, Center for Systems Biology, The University of Texas at Dallas, 800 West Campbell Road, Richardson, Texas 75080, United States of America
⨯
Neha Kashyap,

Affiliations Bioengineering Department, The University of Texas at Dallas, 800 West Campbell Road, Richardson, Texas 75080, United States of America, Center for Systems Biology, The University of Texas at Dallas, 800 West Campbell Road, Richardson, Texas 75080, United States of America
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Michael A. White,

Affiliation Department of Cell Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, Texas 75390, United States of America
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Leonidas Bleris

* E-mail: bleris@utdallas.edu

Affiliations Bioengineering Department, The University of Texas at Dallas, 800 West Campbell Road, Richardson, Texas 75080, United States of America, Center for Systems Biology, The University of Texas at Dallas, 800 West Campbell Road, Richardson, Texas 75080, United States of America, Electrical Engineering Department, The University of Texas at Dallas, 800 West Campbell Road, Richardson, Texas 75080, United States of America
⨯

Exploiting the CRISPR/Cas9 PAM Constraint for Single-Nucleotide Resolution Interventions

Yi Li,
Saurabh Mendiratta,
Kristina Ehrhardt,
Neha Kashyap,
Michael A. White,
Leonidas Bleris

Published: January 20, 2016
https://doi.org/10.1371/journal.pone.0144970

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CRISPR
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Dideoxy DNA sequencing
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Point mutation
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